华南农业大学学报
華南農業大學學報
화남농업대학학보
JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY
2015年
1期
91-95
,共5页
李秋静%卢广超%薛立%田茂涛%林敏%林婧庭
李鞦靜%盧廣超%薛立%田茂濤%林敏%林婧庭
리추정%로엄초%설립%전무도%림민%림청정
绿化树%幼苗%叶绿素荧光参数%臭氧胁迫%干旱胁迫
綠化樹%幼苗%葉綠素熒光參數%臭氧脅迫%榦旱脅迫
녹화수%유묘%협록소형광삼수%취양협박%간한협박
afforestation tree%seedling%chlorophyll fluorescence parameter%ozone stress%drought stress
目的了解尖叶杜英Elaeocarpus apiculatus、米老排Mytilaria laosensis和藜蒴Castanopsis fissa的抗臭氧和干旱生理机制,为华南地区筛选抗逆树种提供参考。方法以3种幼苗为研究对象,进行人工臭氧熏气和干旱处理后,测定其叶绿素荧光参数时间动态。结果和结论随着胁迫处理时间的延长最小初始荧光( F0)先升后降, PSⅡ最大光化学效率(Fv/Fm)保持稳定;臭氧胁迫的PSⅡ实际光量子效率Y(Ⅱ)先降后升,其余处理的保持稳定;尖叶杜英的非光化学淬灭( NPQ)保持稳定,其余幼苗的先升后降。主成分分析表明,幼苗抗臭氧胁迫和干旱胁迫的能力为:黎朔>尖叶杜英>米老排,幼苗抗臭氧和干旱交叉胁迫的能力为:尖叶杜英>黎朔>米老排。总体来看,臭氧胁迫和干旱胁迫下,3种幼苗能够启动光保护作用的调节机制,具有一定的抗臭氧或干旱的能力。
目的瞭解尖葉杜英Elaeocarpus apiculatus、米老排Mytilaria laosensis和藜蒴Castanopsis fissa的抗臭氧和榦旱生理機製,為華南地區篩選抗逆樹種提供參攷。方法以3種幼苗為研究對象,進行人工臭氧熏氣和榦旱處理後,測定其葉綠素熒光參數時間動態。結果和結論隨著脅迫處理時間的延長最小初始熒光( F0)先升後降, PSⅡ最大光化學效率(Fv/Fm)保持穩定;臭氧脅迫的PSⅡ實際光量子效率Y(Ⅱ)先降後升,其餘處理的保持穩定;尖葉杜英的非光化學淬滅( NPQ)保持穩定,其餘幼苗的先升後降。主成分分析錶明,幼苗抗臭氧脅迫和榦旱脅迫的能力為:黎朔>尖葉杜英>米老排,幼苗抗臭氧和榦旱交扠脅迫的能力為:尖葉杜英>黎朔>米老排。總體來看,臭氧脅迫和榦旱脅迫下,3種幼苗能夠啟動光保護作用的調節機製,具有一定的抗臭氧或榦旱的能力。
목적료해첨협두영Elaeocarpus apiculatus、미로배Mytilaria laosensis화려삭Castanopsis fissa적항취양화간한생리궤제,위화남지구사선항역수충제공삼고。방법이3충유묘위연구대상,진행인공취양훈기화간한처리후,측정기협록소형광삼수시간동태。결과화결론수착협박처리시간적연장최소초시형광( F0)선승후강, PSⅡ최대광화학효솔(Fv/Fm)보지은정;취양협박적PSⅡ실제광양자효솔Y(Ⅱ)선강후승,기여처리적보지은정;첨협두영적비광화학쉬멸( NPQ)보지은정,기여유묘적선승후강。주성분분석표명,유묘항취양협박화간한협박적능력위:려삭>첨협두영>미로배,유묘항취양화간한교차협박적능력위:첨협두영>려삭>미로배。총체래간,취양협박화간한협박하,3충유묘능구계동광보호작용적조절궤제,구유일정적항취양혹간한적능력。
Objective] This study was conducted to demonstrate the fluorescent physiology of ozone resist -ance and drought physiology mechanism in the seedlings of three native tree species and provide reference for screening stress-resistant tree species in South China .[Method] Seedlings of Elaeocarpus apiculatus, Mytilaria laosensis and Castanopsis fissa were treated with ozone fumigation and drought condition , and their chlorophyll fluorescence parameters were determined .[Result and conclusion] The results showed that with increasing treatment time , minimal initial fluorescence ( F0 ) of the three seedling types increased and then decreased , and their PSⅡmaximum photochemical efficiency ( Fv/Fm ) remained stable; active quantum efficiency of PSⅡ[ Y (Ⅱ) ] treated with ozone stress decreased , followed by an increase and other treatments remained stable;non-photochemical quenching ( NPQ) of E.apiculatus seedlings remained sta-ble and other seedling types increased and then decreased .Principal component analyses showed that re-sistance of the three seedling types to ozone stress and drought stress was C.fissa >E.apiculatus >M. laosensis, whereas their resistance to intercross stress of ozone and drought was E.apiculatus >C.fissa >M.laosensis.In general, the three seedling types have a certain ability to resist ozone or drought by start -ing an adjustment mechanism of photoprotection under ozone stress or drought stress .