临床神经外科杂志
臨床神經外科雜誌
림상신경외과잡지
JOURNAL OF CLINICAL NEUROSURGERY
2015年
1期
43-47
,共5页
杨阳%王强%满明昊%李玉骞%李立宏
楊暘%王彊%滿明昊%李玉鶱%李立宏
양양%왕강%만명호%리옥건%리립굉
脓毒症%凋亡%胰岛素生长因子1%大鼠模型
膿毒癥%凋亡%胰島素生長因子1%大鼠模型
농독증%조망%이도소생장인자1%대서모형
sepsis%cecal ligation and puncture%apoptosis%insulin-like growth factor-1%rat model
目的：探讨脓毒症大鼠模型胰岛素生长因子1（IGF-1）表达的变化及影响。方法采用盲肠结扎穿孔法（CLP）制作脓毒症大鼠模型，应用免疫荧光染色观察皮层IGF-1阳性细胞， western blot法检测IGF-1和caspase-3蛋白表达，TUNEL染色观察脑神经元凋亡。在脓毒症模型基础上，给予侧脑室定位注射IGF-1或生理盐水，相同方法检测caspase-3蛋白表达及脑神经元凋亡。结果与假手术组相比，脓毒症组大鼠皮层IGF-1阳性细胞数明显减少，caspase-3表达增高，IGF-1表达减低，神经元凋亡增加（均P＜0．05）。侧脑室注射IGF-1后，caspase-3表达和神经元凋亡较假手术组无明显变化；而侧脑室给予生理盐水大鼠caspase-3表达和神经元凋亡与脓毒症组相似。结论脓毒症大鼠的脑皮层IGF-1表达明显减低，细胞凋亡增多。给予外源性IGF-1后，细胞凋亡减少。提示IGF-1可能通过抑制caspase-3的上调对脓毒症大鼠起到脑保护作用。
目的：探討膿毒癥大鼠模型胰島素生長因子1（IGF-1）錶達的變化及影響。方法採用盲腸結扎穿孔法（CLP）製作膿毒癥大鼠模型，應用免疫熒光染色觀察皮層IGF-1暘性細胞， western blot法檢測IGF-1和caspase-3蛋白錶達，TUNEL染色觀察腦神經元凋亡。在膿毒癥模型基礎上，給予側腦室定位註射IGF-1或生理鹽水，相同方法檢測caspase-3蛋白錶達及腦神經元凋亡。結果與假手術組相比，膿毒癥組大鼠皮層IGF-1暘性細胞數明顯減少，caspase-3錶達增高，IGF-1錶達減低，神經元凋亡增加（均P＜0．05）。側腦室註射IGF-1後，caspase-3錶達和神經元凋亡較假手術組無明顯變化；而側腦室給予生理鹽水大鼠caspase-3錶達和神經元凋亡與膿毒癥組相似。結論膿毒癥大鼠的腦皮層IGF-1錶達明顯減低，細胞凋亡增多。給予外源性IGF-1後，細胞凋亡減少。提示IGF-1可能通過抑製caspase-3的上調對膿毒癥大鼠起到腦保護作用。
목적：탐토농독증대서모형이도소생장인자1（IGF-1）표체적변화급영향。방법채용맹장결찰천공법（CLP）제작농독증대서모형，응용면역형광염색관찰피층IGF-1양성세포， western blot법검측IGF-1화caspase-3단백표체，TUNEL염색관찰뇌신경원조망。재농독증모형기출상，급여측뇌실정위주사IGF-1혹생리염수，상동방법검측caspase-3단백표체급뇌신경원조망。결과여가수술조상비，농독증조대서피층IGF-1양성세포수명현감소，caspase-3표체증고，IGF-1표체감저，신경원조망증가（균P＜0．05）。측뇌실주사IGF-1후，caspase-3표체화신경원조망교가수술조무명현변화；이측뇌실급여생리염수대서caspase-3표체화신경원조망여농독증조상사。결론농독증대서적뇌피층IGF-1표체명현감저，세포조망증다。급여외원성IGF-1후，세포조망감소。제시IGF-1가능통과억제caspase-3적상조대농독증대서기도뇌보호작용。
Objective To explore the change of insulin-like growth factor-1( IGF-1) expression and its effects on cerebral cortex of rat sepsis model .Methods The rat model of sepsis was established by cecal ligation and puncture ( CLP ) .The IGF-1 positive cells were observed by immunofluorescent staining .IGF-1 and caspase-3 expression was determined by Western blot . TUNEL staining was used to assess neuron apoptosis .Then septic rats were treated with IGF-1 or saline by lateral ventricle injection .The expression of caspase-3 was evaluated by western blot . Neuron apoptosis was assessed by TUNEL staining .Results Compared with the sham-operated group, IGF-1 positive cells in cerebral cortex in the sepsis group decreased obviously .Besides, IGF-1 expression decreased , caspase-3 expression and neuron apoptosis increased (all P<0.05). When IGF-1 was given , caspase-3 expression and cell apoptosis was similar to the sham-operated group, while in the saline-treated group, the results were similar to the sepsis group .Conclusion During sepsis , IGF-1 expression in rat cerebral cortex obviously decreased and neuron apoptosis increased.After treated with exogenous IGF-1, cell apoptosis decreased .These findings suggested that IGF-1 may protect rat brain from sepsis by inhibiting the up-regulation of caspase-3.
