CAJ | 학술논문

研究了温度、pH 值、溶氧量(以转速表征)、金属离子浓度等因素对氧化亚铁硫杆菌的生长状况及体内 ATP 酶活力的影响,确定了最适的培养条件.通过对比培养前与培养后微生物扫描电镜(SEM),探讨环境胁迫对微生物表面形貌和菌体生长的影响.结果表明,初始 pH 2.0,温度30℃,转速150 r/min,氧化亚铁硫杆菌培养60 h,酶的活性较高,其中最大值为2.8 U/mL,金属离子 Zn2+、Cu2+、Mg2+会降低微生物 ATP酶的活性,当 Zn2+浓度小于0.03 mol/L,Cu2+浓度小于0.08 mol/L,Mg2+浓度小于0.04 mol/L时,对微生物的抑制作用不明显.初始 pH=2.0,温度30℃,转速150 r/min,培养嗜酸氧化亚铁硫杆菌,与对照菌株相比,菌体形状大小没有明显变化,菌体数量显著增加,菌体表面有生物膜形成.
연구료온도、pH 치、용양량(이전속표정)、금속리자농도등인소대양화아철류간균적생장상황급체내 ATP 매활력적영향,학정료최괄적배양조건.통과대비배양전여배양후미생물소묘전경(SEM),탐토배경협박대미생물표면형모화균체생장적영향.결과표명,초시 pH 2.0,온도30℃,전속150 r/min,양화아철류간균배양60 h,매적활성교고,기중최대치위2.8 U/mL,금속리자 Zn2+、Cu2+、Mg2+회강저미생물 ATP매적활성,당 Zn2+농도소우0.03 mol/L,Cu2+농도소우0.08 mol/L,Mg2+농도소우0.04 mol/L시,대미생물적억제작용불명현.초시 pH=2.0,온도30℃,전속150 r/min,배양기산양화아철류간균,여대조균주상비,균체형상대소몰유명현변화,균체수량현저증가,균체표면유생물막형성.
The effect of some factors on the growth and ATP-ase activity of Acidithiobacillus ferrooxi-dans,such as solution temperature,pH,dissolved oxygen and the concentration of metal ions is studied. The influence of environmental condition on microbial surface and the growth of Acidithiobacillus ferrooxi-dans is investigated by the scanning electron microscope(SEM)of per-cultivated and post-cultivated Acidi-thiobacillus ferrooxidans .The results show that under the condition of initial pH 2.0,temperature 30 ℃, speed 150 r/min,cultivated time 60 h,the ATP-ase activity becomes higher,and the largest ATP-ase activ-ity is 2.8 U/mL,Zn2+,Cu2+ and Mg2+ can reduce ATP-ase activity,however,when the concentration of Zn2+,Cu2+ and Mg2+ is less than 0.03,0.08 and 0.04 mol/L respectively,the inhibitory effect is not obvi-ous.The scanning electron microscope(SEM)of per-cultivated and post-cultivated Acidithiobacillus ferro-oxidans show that the number of Acidithiobacillus ferrooxidans increase remarkably,biofilms grew in the surface of Acidithiobacillus ferrooxidans,appreciable change is not found in the shape size of Acidithiobacil-lus ferrooxidans.