现代肿瘤医学
現代腫瘤醫學
현대종류의학
JOURNAL OF MODERN ONCOLOGY
2015年
2期
177-180
,共4页
肺腺癌%PD-L1%凋亡%RNA
肺腺癌%PD-L1%凋亡%RNA
폐선암%PD-L1%조망%RNA
lung adenocarcinoma%PD-L1%apoptosis%RNAi
目的:沉默肺癌细胞系A549的程序性死亡配体l(programmed death ligand 1,PD-L1)基因,观察其对A549细胞增殖和凋亡的影响.方法:构建PD-L1 shRNA重组质粒pGPU6/PD-L1,并应用脂质体转染法将其转染入A549细胞.RT-PCR法检测PD-L1基因的表达;Western blotting法检测PD-L1蛋白的表达;MTT法检测pGPU6/PD-L1对A549细胞增殖的影响;Annexin Ⅴ-FITC/PI双染法检测pGPU6/PD-L1对A549细胞凋亡的影响.结果:成功将pGPU6/PD-L1转染入A549细胞;RT-PCR结果显示pGPU6/PD-L1使A549细胞PD-L1基因表达水平降低;Western blotting结果显示pGPU6/PD-L1转染A549细胞使PD-L1蛋白表达减少;MTT结果显示pGPU6/PD-L1能够抑制A549细胞增殖;Annexin Ⅴ-FITC/PI双染法检测结果显示pGPU6/PD-L1能够诱导A549细胞凋亡.结论:pGPU6/PD-L1能够下调肺癌细胞A549 PD-L1的表达,抑制细胞增殖,并诱导细胞凋亡.
目的:沉默肺癌細胞繫A549的程序性死亡配體l(programmed death ligand 1,PD-L1)基因,觀察其對A549細胞增殖和凋亡的影響.方法:構建PD-L1 shRNA重組質粒pGPU6/PD-L1,併應用脂質體轉染法將其轉染入A549細胞.RT-PCR法檢測PD-L1基因的錶達;Western blotting法檢測PD-L1蛋白的錶達;MTT法檢測pGPU6/PD-L1對A549細胞增殖的影響;Annexin Ⅴ-FITC/PI雙染法檢測pGPU6/PD-L1對A549細胞凋亡的影響.結果:成功將pGPU6/PD-L1轉染入A549細胞;RT-PCR結果顯示pGPU6/PD-L1使A549細胞PD-L1基因錶達水平降低;Western blotting結果顯示pGPU6/PD-L1轉染A549細胞使PD-L1蛋白錶達減少;MTT結果顯示pGPU6/PD-L1能夠抑製A549細胞增殖;Annexin Ⅴ-FITC/PI雙染法檢測結果顯示pGPU6/PD-L1能夠誘導A549細胞凋亡.結論:pGPU6/PD-L1能夠下調肺癌細胞A549 PD-L1的錶達,抑製細胞增殖,併誘導細胞凋亡.
목적:침묵폐암세포계A549적정서성사망배체l(programmed death ligand 1,PD-L1)기인,관찰기대A549세포증식화조망적영향.방법:구건PD-L1 shRNA중조질립pGPU6/PD-L1,병응용지질체전염법장기전염입A549세포.RT-PCR법검측PD-L1기인적표체;Western blotting법검측PD-L1단백적표체;MTT법검측pGPU6/PD-L1대A549세포증식적영향;Annexin Ⅴ-FITC/PI쌍염법검측pGPU6/PD-L1대A549세포조망적영향.결과:성공장pGPU6/PD-L1전염입A549세포;RT-PCR결과현시pGPU6/PD-L1사A549세포PD-L1기인표체수평강저;Western blotting결과현시pGPU6/PD-L1전염A549세포사PD-L1단백표체감소;MTT결과현시pGPU6/PD-L1능구억제A549세포증식;Annexin Ⅴ-FITC/PI쌍염법검측결과현시pGPU6/PD-L1능구유도A549세포조망.결론:pGPU6/PD-L1능구하조폐암세포A549 PD-L1적표체,억제세포증식,병유도세포조망.
