CAJ | 학술논문

针对二苯并噻吩高效降解菌Pseudomonas sp.LKY-5降解过程中产生表面活性剂的现象，进行碳源优化，将提取分离出的表面活性剂进行化学组分分析和理化性质测定，考察温度、pH、无机离子对其表面活性稳定性的影响。结果表明，花生油为Pseudomonas sp.LKY-5产生表面活性剂的最佳碳源，产生的表面活性剂为鼠李糖脂，产量为0.15 g？L，临界胶束浓度（CMC）为180 mg？L，亲水亲油平衡值（HLB）为12.3，对柴油24 h的乳化能力达61％。该表面活性剂在温度30～80℃、pH 6～13的条件下表面活性稳定，能够耐受200 g？L的NaCl或 MgCl2以及20 g？L的CaCl2，稳定性能良好。
침대이분병새분고효강해균Pseudomonas sp.LKY-5강해과정중산생표면활성제적현상，진행탄원우화，장제취분리출적표면활성제진행화학조분분석화이화성질측정，고찰온도、pH、무궤리자대기표면활성은정성적영향。결과표명，화생유위Pseudomonas sp.LKY-5산생표면활성제적최가탄원，산생적표면활성제위서리당지，산량위0.15 g？L，림계효속농도（CMC）위180 mg？L，친수친유평형치（HLB）위12.3，대시유24 h적유화능력체61％。해표면활성제재온도30～80℃、pH 6～13적조건하표면활성은정，능구내수200 g？L적NaCl혹 MgCl2이급20 g？L적CaCl2，은정성능량호。
The Pseudomonas sp. LKY-5 produces biosurfactant during biodegradation process of dibenzothiophene.This work optimizes the carbon sources for biosurfactant production,verifies the bio-surfactant by thin-layer chromatography,and then determines its physicochemical properties,and inves-tigates the influence of temperature,pH and inorganic ions on the active stability of biosurfactant.The results show that the biosurfactant produced is rhamnolipid,and peanut oil is the optimal carbon source for the production of biosurfactant with a yield of 0.1 5 g?L.The critical micelle concentration (CMC) and hydrophile-lipophile balance (HLB)value of biosurfactant are 180 mg?L and 12.3,respectively.Twenty-four hours diesel emulsification ability is 6 1%. The biosurfactant is stable at conditions of a temperature of 30—80 ℃,a pH of 6—13,and can tolerate the environment of 200 g?L NaCl (or MgCl2) or 20 g?L CaCl2.