CAJ | 학술논문

以秀丽新杆线虫作为模式生物,研究 w(左卡尼汀)=0.1%,w(曲美他嗪)=0.0005%及两种单药的复方药物复方左卡尼汀对连续三代线虫寿命与生殖能力的影响,并对线虫总脂肪进行油红 O 染色和气相色谱分析.结果表明：w (左卡尼汀)=0.1%,w (曲美他嗪)=0.0005%及复方左卡尼汀对连续三代线虫的寿命均影响较小；左卡尼汀和曲美他嗪对亲代、F1代和 F2代线虫的生殖能力均产生负面影响；复方左卡尼汀对线虫的生殖能力具有保护作用；限制饮食可减少线虫的产卵量,并与线虫的脂肪累积有关；左卡尼汀和曲美他嗪可减少线虫体内的脂肪累积与总脂肪酸的体积分数,复方左卡尼汀对线虫体内脂肪累积和总脂肪酸的体积分数影响较小.
이수려신간선충작위모식생물,연구 w(좌잡니정)=0.1%,w(곡미타진)=0.0005%급량충단약적복방약물복방좌잡니정대련속삼대선충수명여생식능력적영향,병대선충총지방진행유홍 O 염색화기상색보분석.결과표명：w (좌잡니정)=0.1%,w (곡미타진)=0.0005%급복방좌잡니정대련속삼대선충적수명균영향교소；좌잡니정화곡미타진대친대、F1대화 F2대선충적생식능력균산생부면영향；복방좌잡니정대선충적생식능력구유보호작용；한제음식가감소선충적산란량,병여선충적지방루적유관；좌잡니정화곡미타진가감소선충체내적지방루적여총지방산적체적분수,복방좌잡니정대선충체내지방루적화총지방산적체적분수영향교소.
Taking Caenorhabditis elegans worm as a model organism,we examined the effects of L-carnitine,trimetazidine,compound L-carnitine on the lifespan and reproductivity of the worms. Our results show that w (L-carnitine )= 0.1%,w (trimetazidine )= 0.000 5% and compound L-carnitine did not change the lifespan of C.elegans .On the other hand,w (L-carnitine)= 0.1%, w(trimetazidine)=0.000 5% significantly reduced the brood size in parental generation,F1 and F2. However,compound L-carnitine has no effect on the reproductivity of the worms.Worms undergoing DR utilize fat,and diminish reproductive output.That is to say,the worms with restricteddietary intake have reduced broods.Our results indicate that w(L-carnitine)=0.1% and w(trimetazidine)=0.000 5% reduce both fat accumulation and overall fatty acid.However,compound L-carnitine has no effect on the overall fat and fat acid.