CAJ | 학술논문

利用5种发根农杆菌侵染不同花生外植体，对比其发根诱导率，分析寻找利于花生转化的外植体、花生品种及发根农杆菌。结果表明，农杆菌94022诱导发根率较其它发根农杆菌高；后期继代培养中子叶诱导的发根易于培养，存活率高，生长状态好；在用培养后的子叶为外植体时，花育20较其它品种发根诱导率高。
이용5충발근농간균침염불동화생외식체，대비기발근유도솔，분석심조리우화생전화적외식체、화생품충급발근농간균。결과표명，농간균94022유도발근솔교기타발근농간균고；후기계대배양중자협유도적발근역우배양，존활솔고，생장상태호；재용배양후적자협위외식체시，화육20교기타품충발근유도솔고。
Five kinds of Agrobacterium rhizogenes were used to infect peanut explants and the root induc-tion rates were contrasted to find the suitable explants, peanut varieties and A.rhizogenes for peanut transfor-mation.The results showed that A.rhizogenes 94022 had higher root induction rate than the others.The hairy roots induced from cotyledon were easy to cultivate in the successive transfer culture with higher survival rate and good growth condition.With cultured cotyledon as explants, Huayu 20 had higher root induction rate than other peanut varieties.