CAJ | 학술논문

以红旗16为受体、明恢63为供体，经杂交和回交得到BC4 F1。利用643对SSR分子标记对所构建的抽穗期基因早晚池和亲本进行多态性筛选，得到4对多态性标记。将筛选出的杂合单株进行自交，获得BC4 F2，并从中筛选出抽穗期分离较明显的群体进行田间表型调查和基因型检测。发现目标基因与标记PSM391连锁，位于第7染色体长臂末端，命名为Hd7m。在标记PSM391与第7染色体末端之间合成10对新SSR标记，其中多态性标记RM22156与目标基因相距4．1 cM。该结果为Hd7m基因的精细定位、基因克隆和分子标记辅助育种奠定了基础。
이홍기16위수체、명회63위공체，경잡교화회교득도BC4 F1。이용643대SSR분자표기대소구건적추수기기인조만지화친본진행다태성사선，득도4대다태성표기。장사선출적잡합단주진행자교，획득BC4 F2，병종중사선출추수기분리교명현적군체진행전간표형조사화기인형검측。발현목표기인여표기PSM391련쇄，위우제7염색체장비말단，명명위Hd7m。재표기PSM391여제7염색체말단지간합성10대신SSR표기，기중다태성표기RM22156여목표기인상거4．1 cM。해결과위Hd7m기인적정세정위、기인극륭화분자표기보조육충전정료기출。
Using Hongqi 16 as the receptor and Minghui 63 as the donor, a BC4 F1 segregation population was developed through cross between the two parents and backcross with Hongqi 16.The early heading gene pool, late heading gene pool, and the parents were analyzed with 643 pairs of SSR markers, and 4 pairs of polymorphic markers were obtained.BC4 F2 populations derived from inbreeding of BC4 F1 hybrid plants with obvious segregation phenotype were selected for field investigation and genotype detection.The results showed that the target gene, named Hd7m, was linked to SSR marker PSM391, which located at the end of chromo-some 7.Based on the targeted interval, 10 pairs of new SSR markers were synthesized.Among which, the polymorphic marker RM22156 was apart from Hd7m of 4.1 cM.The results laid foundations for fine mapping, gene cloning and molecular marker assisted breeding of Hd7m.