CAJ | 학술논문

利用ISSR标记和叶绿体DNA trnL-trnF非编码区分析36种葡萄种质的遗传多样性和系统发育关系.13个ISSR引物共产生了63条带,平均有效等位基因数、Nei’s 基因多样性和香农信息指数分别是1．5827,0．3463和0．5172.基于UPGMA的ISSR分析将葡萄种质材料分为6个主要的进化枝.葡萄在trnL-trnF区域有较高的遗传多样性水平(Hd=0．667,Pi=0．00206).由NJ,ML和贝叶斯分析得到的进化树都没有表现出不同地区葡萄品种与地域之间的地理相关性.中性检验葡萄的进化历史过程中发生过种群扩张事件.葡萄种质资源的保护应以就地保护为主.本研究为葡萄种质资源的理论和应用研究提供了有价值的信息.
이용ISSR표기화협록체DNA trnL-trnF비편마구분석36충포도충질적유전다양성화계통발육관계.13개ISSR인물공산생료63조대,평균유효등위기인수、Nei’s 기인다양성화향농신식지수분별시1．5827,0．3463화0．5172.기우UPGMA적ISSR분석장포도충질재료분위6개주요적진화지.포도재trnL-trnF구역유교고적유전다양성수평(Hd=0．667,Pi=0．00206).유NJ,ML화패협사분석득도적진화수도몰유표현출불동지구포도품충여지역지간적지리상관성.중성검험포도적진화역사과정중발생과충군확장사건.포도충질자원적보호응이취지보호위주.본연구위포도충질자원적이론화응용연구제공료유개치적신식.
Nuclear ISSRs and cpDNA non-coding trnL-trnF region were used to analyse the genetic diversity and phylogenetic relationships of 36 grape accessions of Vitis vinifera L. Thirteen ISSR primers produced 63 bands in total,and the average values of the number of effective alleles,Nei’ s gene diversity,and Shannon’ s information index were 1. 5827,0. 3463,and 0. 5172,respectively. The ISSR UPGMA cluster analysis showed six major clusters. Vitis vinifera showed a high level of genetic diversity (Hd=0. 667,Pi=0. 00206) in the trnL-trnF region. The relationships inferred from the NJ,ML and BI analyses did not show geographical corre-spondence. Selective neutrality tests indicated that there had been a population expansion in their evolutionary history. In-situ conservation should be the most important approach. These results will be useful for the exploi-tation of grape germplasm in basic and applied research.