CAJ | 학술논문

目的：建立高效液相色谱法测定乌金胶囊中毛蕊异黄酮含量。方法色谱柱为ZORBA Bonus‐RP （4．6 mm ×260 mm ，5μm）分析柱，流动相乙腈‐水（含0．1％甲酸）梯度洗脱，柱温25℃，检测波长为260 nm ，流速1．0 ml／min ，进样量10μl。结果毛蕊异黄酮在7．4～117．6μg／m l （ r＝1．000）浓度范围内呈良好线性关系，其回归方程为Y ＝34．002 X －3．4512，回收率为95．24％（RSD＝2．10％）。6批乌金胶囊中异黄酮含量在9．26～23．14μg／g之间。结论该方法简便、快速、准确，可用于乌金胶囊的质量控制。
목적：건립고효액상색보법측정오금효낭중모예이황동함량。방법색보주위ZORBA Bonus‐RP （4．6 mm ×260 mm ，5μm）분석주，류동상을정‐수（함0．1％갑산）제도세탈，주온25℃，검측파장위260 nm ，류속1．0 ml／min ，진양량10μl。결과모예이황동재7．4～117．6μg／m l （ r＝1．000）농도범위내정량호선성관계，기회귀방정위Y ＝34．002 X －3．4512，회수솔위95．24％（RSD＝2．10％）。6비오금효낭중이황동함량재9．26～23．14μg／g지간。결론해방법간편、쾌속、준학，가용우오금효낭적질량공제。
Objective To explore method for determination of calycosin contents in Wujin capsule by HPLC .Methods The sample was studied on ZORBA Bonus‐RP (4 .6 mm × 260 mm ,5 μm) column ,the mobile phase consisted of acetonitrile and H2O‐0.1% HCOOH (gradient elution) ,the temperature of column was 25 ℃ ,the UV detection wavelength was set at 260 nm , flow speed was 1 .0 ml/min and injection volume was 10 μl .Results The calycosin calibration curve was at the range of 7 .4‐117 .6 μg/ml (r=1 .000) ,with their regression function being Y=34 .002 X-3 .451 2 .The recovery of calycosin was 95 .24%(RSD=2 .10% ) .6 batches of Wujin capsule calycosin contents were 9 .26‐23 .14μg/g .Conclusion HPLC was an accuracy and simple method for determining the content of calycosin in Wujin capsule .