CAJ | 학술논문

目的：通过高效液相色谱‐飞行时间质谱（HPLC‐TOF‐MS）联用技术，对中药复方芎归汤中的主要化学成分进行有效鉴定。方法色谱分离采用资生堂 CAPCELL PAK C18反相柱（100 mm ×3．0 mm ，3μm），流动相组成分别为0．1％甲酸水溶液和乙腈，梯度洗脱，流速为0．4 ml／min；质谱定性采用飞行时间质谱，正离子模式扫描。结果在优化的液质联用条件下，通过飞行时间质谱鉴定了芎归汤中47种主要成分，并对其药材来源进行了归属。结论通过 HPLC‐TOF‐MS联用技术，为鉴定芎归汤中的化学成分建立起了一种快速、高效的分析方法。
목적：통과고효액상색보‐비행시간질보（HPLC‐TOF‐MS）련용기술，대중약복방궁귀탕중적주요화학성분진행유효감정。방법색보분리채용자생당 CAPCELL PAK C18반상주（100 mm ×3．0 mm ，3μm），류동상조성분별위0．1％갑산수용액화을정，제도세탈，류속위0．4 ml／min；질보정성채용비행시간질보，정리자모식소묘。결과재우화적액질련용조건하，통과비행시간질보감정료궁귀탕중47충주요성분，병대기약재래원진행료귀속。결론통과 HPLC‐TOF‐MS련용기술，위감정궁귀탕중적화학성분건립기료일충쾌속、고효적분석방법。
Objective To analyze chemical constituents of Xionggui Decotion by rapid‐resolution (high performance) liquid chromatography‐time of flight mass spectrometry (HPLC‐TOF‐MS) .Methods A Shelloseido column(100 mm × 3 .0 mm ,3 μm)was used to separate .The mobile phase consisted of water containing 0 .1% methane acid and acetonitrile was used as gradient elute .The flow rate was 0 .4 ml/min .TOF‐MS was applied for qualitative analysis under positive ion mode . Results Under LC/MS condition ,47 major constituents in Xionggui Decotion were identified by time of flight mass spectrome‐try and structure‐relevant fragment ions .Conclusion A simple and reliable method using HPLC‐TOF‐MS was established to i‐dentify the chemical constituents of Xionggui Decotion .