CAJ | 학술논문

目的：考察百蕊草提取物对免疫球蛋白A肾病（IgAN）模型大鼠的治疗效果，以明确有效部位。方法采用水煎提取百蕊草，AB‐8大孔树脂吸附，乙醇梯度洗脱制备各部位。给予大鼠口服牛血清蛋白（400mg／kg），皮下注射蓖麻油、CCl4联合尾静脉注射脂多糖造模。从第8周起，对阳性组[雷公藤多苷（LGT），10mg／kg]、水提取物组（TT，1．0g／kg）、水溶性部位组（TTW，0．4g／kg）、极性部位组（TT20，0．4g／kg）和中等极性部位组大鼠（TT50，0．4g／kg）每天灌胃给药，空白组和模型组给予生理盐水（n＝8），连续5周。观察各组大鼠24h尿量、24h尿蛋白、尿沉渣红细胞计数，实验结束后进行血液生化指标检测、免疫荧光检测、肾组织的病理学检查。结果与空白组相比，模型组大鼠的24h尿蛋白、尿沉渣中红细胞数量、血清中肌酐、尿素氮水平均显著升高。与模型组相比，TT组和TT50组的24h尿蛋白（P＜0．05）、尿沉渣中红细胞数量（P＜0．01）、血清肌酐和尿素氮水平（P＜0．05）降低。各给药组肾组织系膜区的免疫复合物沉积减少、肾小球增大、系膜基质增生、肾小管的损伤状况改善。结论百蕊草水提物及其中等极性部位对IgA肾病大鼠具有良好的治疗作用，其效果优于阳性对照雷公藤多苷。
목적：고찰백예초제취물대면역구단백A신병（IgAN）모형대서적치료효과，이명학유효부위。방법채용수전제취백예초，AB‐8대공수지흡부，을순제도세탈제비각부위。급여대서구복우혈청단백（400mg／kg），피하주사비마유、CCl4연합미정맥주사지다당조모。종제8주기，대양성조[뢰공등다감（LGT），10mg／kg]、수제취물조（TT，1．0g／kg）、수용성부위조（TTW，0．4g／kg）、겁성부위조（TT20，0．4g／kg）화중등겁성부위조대서（TT50，0．4g／kg）매천관위급약，공백조화모형조급여생리염수（n＝8），련속5주。관찰각조대서24h뇨량、24h뇨단백、뇨침사홍세포계수，실험결속후진행혈액생화지표검측、면역형광검측、신조직적병이학검사。결과여공백조상비，모형조대서적24h뇨단백、뇨침사중홍세포수량、혈청중기항、뇨소담수평균현저승고。여모형조상비，TT조화TT50조적24h뇨단백（P＜0．05）、뇨침사중홍세포수량（P＜0．01）、혈청기항화뇨소담수평（P＜0．05）강저。각급약조신조직계막구적면역복합물침적감소、신소구증대、계막기질증생、신소관적손상상황개선。결론백예초수제물급기중등겁성부위대IgA신병대서구유량호적치료작용，기효과우우양성대조뢰공등다감。
Objective To evaluate the therapeutic effects of Thesium chinensis Turcz extracts on IgA nephropathy (IgAN) rats .Methods The hot water extracted solution of Thesium chinensis Turcz flow through the AB‐8 macroreticular res‐in column repeatedly ,then being eluted with different contents of ethanol to get four part of extract .The IgAN model of SD rats were established by oral intake of bovine serum albumin(BSA ,400 mg/kg) together with injection of lipopolysaccharide(LPS) and CCl4 .Beginning with 8th week ,the positive group(LGT ,10 mg/kg) ,water extract group(TT ,1 .0 g/kg) ,water soluble group(TTW ,0 .4 g/kg) ,high polarity group(TT20 ,0 .4 g/kg) and medium polarity group(TT50 ,0 .4 g/kg) rats were intragas‐tric administrated drug daily ,the normal group control and model control group rats were given with saline(n=8) .During the next 5 weeks treatment process ,the 24 h urine volume ,24 h urine protein concentration ,and quantity of erythrocyte in urine were observed .The levels of TP ,ALP ,ALT ,AST ,Scr and BUN in serum were determined .The expression of IgA deposition was measured by immunofluorescence staining ,and the pathological changes of kidney tissue were assayed after the therapy process .Results After 5 weeks therapy ,the 24 h urine protein concentration ,the quantity of erythrocyte in urine ,and the level of Scr and BUN of TT and TT50 groups were significantly lower than model control .The fluorescence intensities in the me‐sangial area were weaker than model control .Also ,the degrees of renal capsule expansion and mesangial matrix proliferations , and the kidney tubules damage of TT and TT50 groups were weaker than those of model control .Conclusion The water extract and its medium polarity fraction of Thesium chinensis Turcz show good therapeutic effects on IgAN rats .