CAJ | 학술논문

目的：探讨运动预处理对脑缺血再灌注大鼠血清白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)及肿瘤坏死因子α(TNF-α)含量的影响。方法雄性Sprague-Dawley大鼠24只分为运动预处理组(n=8)、模型组(n=8)、假手术组(n=8)。线栓法制备大鼠大脑中动脉阻塞(MCAO)缺血再灌注模型。再灌注后2 h、24 h分别行神经功能缺损评分。随后取材，HE染色观察大鼠缺血侧脑组织病理形态变化，酶联免疫吸附法检测血清TNF-α、IL-1β及IL-6的含量。结果脑缺血再灌注后24 h，运动预处理组神经功能评分较模型组改善(P<0.05)，血清TNF-α、IL-1β及IL-6含量明显降低(P<0.01)；脑缺血区皮质病理损伤减轻，间质水肿程度减轻，细胞排列较整齐，缺血区变性和坏死的神经元数量明显减少。结论运动预处理可以降低急性脑缺血再灌注大鼠炎症反应，降低神经功能缺损。
목적：탐토운동예처리대뇌결혈재관주대서혈청백세포개소-1β(IL-1β)、백세포개소-6(IL-6)급종류배사인자α(TNF-α)함량적영향。방법웅성Sprague-Dawley대서24지분위운동예처리조(n=8)、모형조(n=8)、가수술조(n=8)。선전법제비대서대뇌중동맥조새(MCAO)결혈재관주모형。재관주후2 h、24 h분별행신경공능결손평분。수후취재，HE염색관찰대서결혈측뇌조직병리형태변화，매련면역흡부법검측혈청TNF-α、IL-1β급IL-6적함량。결과뇌결혈재관주후24 h，운동예처리조신경공능평분교모형조개선(P<0.05)，혈청TNF-α、IL-1β급IL-6함량명현강저(P<0.01)；뇌결혈구피질병리손상감경，간질수종정도감경，세포배렬교정제，결혈구변성화배사적신경원수량명현감소。결론운동예처리가이강저급성뇌결혈재관주대서염증반응，강저신경공능결손。
Objective To investigate the effect of exercise preconditioning on serum level of tumor necrosis factorα(TNF-α), interleu-kin (IL)-1βand IL-6 in rats after cerebral ischemia-reperfusion (I/R). Methods 24 male Sprague-Dawley rats were randomly divided into exercise preconditioning group (n=8), model group (n=8) and sham group (n=8). The middle cerebral arteries were occluded for 120 min and re-perfused. All the rats were evaluated with neurological deficit score 2 hours, 24 hours after I/R. The serum levels of TNF-α, IL-1βand IL-6 was detected with enzyme-linked immunosorbent, and the pathology was observed with HE staining 24 hours after I/R. Results The neurological deficit score decreased in the exercise preconditioning group compared with that in the model group, as well as the serum TNF-α, IL-1βand IL-6 (P<0.05) 24 hours after I/R. The pathological damage and interstitial edema alleviated in cerebral ischemia cortical, and the degeneration and necrosis of the neurons in the ischemic area significantly reduced in the exercise preconditioning group. Conclu-sion Exercise preconditioning may inhibit inflammatory response in I/R rats to protect neurological function from impairment.