CAJ | 학술논문

目的：探讨电针神庭、百会穴对脑缺血再灌注模型大鼠学习记忆能力的影响及其可能机制。方法45只雄性Sprague-Dawley大鼠随机分为假手术组(n=15)、模型组(n=15)和电针组(n=15)。模型组和电针组均采用左侧大脑中动脉缺血(MCAO)再灌注模型。电针组电针神庭、百会穴共7 d。采用Morris水迷宫测试观察大鼠学习记忆能力；尼氏染色观察大鼠海马神经元形态结构变化；Western blotting法检测大鼠左侧海马RhoA蛋白的表达。结果与模型组相比，电针组学习记忆能力改善(P<0.05)，海马神经元损伤减少(P<0.05)，海马组织中RhoA蛋白表达降低(P<0.05)。结论电针能够改善脑缺血再灌注大鼠学习记忆能力，其机制可能与抑制RhoA蛋白表达有关。
목적：탐토전침신정、백회혈대뇌결혈재관주모형대서학습기억능력적영향급기가능궤제。방법45지웅성Sprague-Dawley대서수궤분위가수술조(n=15)、모형조(n=15)화전침조(n=15)。모형조화전침조균채용좌측대뇌중동맥결혈(MCAO)재관주모형。전침조전침신정、백회혈공7 d。채용Morris수미궁측시관찰대서학습기억능력；니씨염색관찰대서해마신경원형태결구변화；Western blotting법검측대서좌측해마RhoA단백적표체。결과여모형조상비，전침조학습기억능력개선(P<0.05)，해마신경원손상감소(P<0.05)，해마조직중RhoA단백표체강저(P<0.05)。결론전침능구개선뇌결혈재관주대서학습기억능력，기궤제가능여억제RhoA단백표체유관。
Objective To explore the effects of electroacupuncture at Shenting (DU24) and Baihui (DU20) on learning and memory abil-ity in the cerebral ischemia-reperfusion rats and its possible mechanism. Methods 45 male Sprague-Dawley rats were randomly divided into sham group (n=15), ischemia group (n=15) and electroacupuncture group (n=15). The latter 2 groups were modeled as focal cerebral isch-emia-reperfusion injury, and the electroacupuncture group received electroacupuncture at Shenting (DU24) and Baihui (DU20) for 7 days. They were tested with Morris Water Maze, observed with Nissl's staining. The protein expression of RhoA was detected with Western blot-ting. Results The learning and memory ability improved in the electroacupuncture group (P<0.05), the injury of the neurons reduced (P<0.05) and the expression of RhoA in hippocampus decreased compared with the ischemia group (P<0.05). Conclusion Electroacupuncture could ameliorate the learning and memory ability in ischemia-reperfusion rats, which may relate with the inhibition of the expression of RhoA in hippocampus.