中国康复理论与实践
中國康複理論與實踐
중국강복이론여실천
CHINESE JOURNAL OF REHABILITATION THEORY & PRACTICE
2015年
1期
1-4
,共4页
艾厚喜%孙芳玲%侯虹丽%张丽%王文
艾厚喜%孫芳玲%侯虹麗%張麗%王文
애후희%손방령%후홍려%장려%왕문
莫诺苷%脑缺血再灌注%转录因子%Wnt信号通路%神经发生素2%Pax6%Tbr2%大鼠
莫諾苷%腦缺血再灌註%轉錄因子%Wnt信號通路%神經髮生素2%Pax6%Tbr2%大鼠
막낙감%뇌결혈재관주%전록인자%Wnt신호통로%신경발생소2%Pax6%Tbr2%대서
morroniside%cerebral ischemia-reperfusion%transcription factors%Wnt signaling%neurogenin 2%Pax 6%Tbr2%rats
目的研究莫诺苷对脑缺血再灌注7 d患侧皮层Wnt信号通路相关转录因子神经发生素2(Ngn2)、Pax6、Tbr2表达的影响。方法健康成年雄性Sprague-Dawley大鼠随机分为假手术组，模型组，莫诺苷小、中、大剂量组，每组3只。线栓法制备大鼠大脑中动脉阻塞模型，造模后3 h予莫诺苷30 mg/kg、90 mg/kg、270 mg/kg每天1次灌胃。Western blotting法分析术后7 d皮层Ngn2、Pax6、Tbr2的表达。结果与假手术组相比，模型组皮层Ngn2表达升高(P<0.05)；与模型组相比，莫诺苷中、高剂量组Ngn2表达明显升高(P<0.01)。模型组皮层Pax6的表达与假手术组无显著性差异，莫诺苷中、高剂量组Pax6表达升高(P<0.05)。各组间Tbr2的表达无显著性差异。结论莫诺苷能增加脑缺血再灌注后皮层Ngn2、Pax6的表达，提示有促进神经发生的作用。
目的研究莫諾苷對腦缺血再灌註7 d患側皮層Wnt信號通路相關轉錄因子神經髮生素2(Ngn2)、Pax6、Tbr2錶達的影響。方法健康成年雄性Sprague-Dawley大鼠隨機分為假手術組，模型組，莫諾苷小、中、大劑量組，每組3隻。線栓法製備大鼠大腦中動脈阻塞模型，造模後3 h予莫諾苷30 mg/kg、90 mg/kg、270 mg/kg每天1次灌胃。Western blotting法分析術後7 d皮層Ngn2、Pax6、Tbr2的錶達。結果與假手術組相比，模型組皮層Ngn2錶達升高(P<0.05)；與模型組相比，莫諾苷中、高劑量組Ngn2錶達明顯升高(P<0.01)。模型組皮層Pax6的錶達與假手術組無顯著性差異，莫諾苷中、高劑量組Pax6錶達升高(P<0.05)。各組間Tbr2的錶達無顯著性差異。結論莫諾苷能增加腦缺血再灌註後皮層Ngn2、Pax6的錶達，提示有促進神經髮生的作用。
목적연구막낙감대뇌결혈재관주7 d환측피층Wnt신호통로상관전록인자신경발생소2(Ngn2)、Pax6、Tbr2표체적영향。방법건강성년웅성Sprague-Dawley대서수궤분위가수술조，모형조，막낙감소、중、대제량조，매조3지。선전법제비대서대뇌중동맥조새모형，조모후3 h여막낙감30 mg/kg、90 mg/kg、270 mg/kg매천1차관위。Western blotting법분석술후7 d피층Ngn2、Pax6、Tbr2적표체。결과여가수술조상비，모형조피층Ngn2표체승고(P<0.05)；여모형조상비，막낙감중、고제량조Ngn2표체명현승고(P<0.01)。모형조피층Pax6적표체여가수술조무현저성차이，막낙감중、고제량조Pax6표체승고(P<0.05)。각조간Tbr2적표체무현저성차이。결론막낙감능증가뇌결혈재관주후피층Ngn2、Pax6적표체，제시유촉진신경발생적작용。
Objective To study the effects of morroniside on the expression of Wnt signaling-related transcription factors neurogenin 2 (Ngn2), Pax6 and Tbr2 in the ischemic ipsilateral cortex 7 days after cerebral ischemia-reperfusion in rats. Methods 15 male Sprague-Dawley rats were randomly divided into sham group (n=3), ischemia group (n=3), and morroniside groups (low, medium and high dosage groups, n=3). The middle cerebral artery were occluded for 30 min, and re-perfused. Morroniside was administered intragastrically once a day at dose of 30 mg/kg, 90 mg/kg and 270 mg/kg 3 hours after operation. The expression of Ngn2, Pax6 and Tbr2 in the ischemic ipsilateral cortex were detected with Western blotting analysis 7 days after operation. Results The expression of Ngn2 increased in the ischemia group compared with the sham group (P<0.05), and it further increased the morroniside groups of medium and high dosage compared with the ischemia group (P<0.01). There was no significant difference between the ischemia group and sham group in the expression of Pax6, while it increased the morroniside groups of medium and high dosage compared with the ischemia group (P<0.01). There was no significant difference among all the groups in the expression of Tbr2. Conclusion Morroniside could increase the expression of Ngn2 and Pax6 in the ischemic ipsilateral cortex 7 days after ischemia-reperfusion in rats, suggesting promoting the neurogenesis after ischemia.
