CAJ | 학술논문

目的：探讨桧木醇（hinokitiol）对人肾透明细胞癌786-O细胞株的增殖抑制作用及诱导凋亡效应，初步阐明其机制。方法：采用CCK-8法检测桧木醇对肾癌786-O细胞增殖的抑制作用；流式细胞术检测细胞凋亡情况；转染EGFP-LC3后镜下观察细胞自噬状态；采用Western blot对细胞Caspase-3剪切体、LC3和P62蛋白表达量进行检测。结果：桧木醇可以显著抑制肾癌786-O细胞增殖，通过激活Caspase途径诱导细胞凋亡。桧木醇诱导肾癌786-O细胞发生自噬，LC3蛋白表达显著上调，而P62蛋白表达下调。结论：桧木醇可显著抑制肾癌786-O细胞的增殖并通过过度激活细胞自噬促进肾癌细胞凋亡。
목적：탐토회목순（hinokitiol）대인신투명세포암786-O세포주적증식억제작용급유도조망효응，초보천명기궤제。방법：채용CCK-8법검측회목순대신암786-O세포증식적억제작용；류식세포술검측세포조망정황；전염EGFP-LC3후경하관찰세포자서상태；채용Western blot대세포Caspase-3전절체、LC3화P62단백표체량진행검측。결과：회목순가이현저억제신암786-O세포증식，통과격활Caspase도경유도세포조망。회목순유도신암786-O세포발생자서，LC3단백표체현저상조，이P62단백표체하조。결론：회목순가현저억제신암786-O세포적증식병통과과도격활세포자서촉진신암세포조망。
Objective: To investigate the effects of hinokitiol on the proliferative inhibition and apoptosis induction in human clear cell renal cancer 786-O cells. Methods:CCK-8 assays were performed to analyze the effects of hinokitiol on the proliferation of 786-O cells. The apoptosis rate was determined by flow cytometry. EGFP-LC3 microscopy assays were performed to assess the autoph-agy flux. Cleaved Caspase-3, LC3, and P62 were detected by Western blot. Results: Hinokitiol could inhibit the proliferation of the 786-O cells and could induce cell apoptosis via Caspase pathway. Hinokitiol induced the autophagy of 786-O cells, increased LC3 ex-pression, and downregulated P62 expression. Conclusion: Hinokitiol can inhibit the proliferation of 786-O cells and can induce cell apoptosis via autophagy induction.