CAJ | 학술논문

在谷胱甘肽功能化的金纳米粒子表面螯合Fe3+离子后,研究了DNA分子在不同pH和盐浓度条件下在粒子表面的吸附和脱附行为。在pH<4.0的条件下,DNA通过所含的磷酸根基团与粒子表面的Fe3+离子产生配位相互作用而吸附到粒子表面,此过程受盐浓度影响不大。当pH>4.0时,由于溶液中氢氧根离子的竞争作用,使DNA的磷酸根基团不能与Fe3+离子配位,此时只能通过静电或氢键作用吸附,但是吸附效率很低。因此,Fe3+离子修饰的金纳米粒子只在低pH条件下可以实现对DNA的高效吸附。这种基于配位作用的吸附效率要高于盐桥作用,而且不会受到溶液中盐浓度的影响。
재곡광감태공능화적금납미입자표면오합Fe3+리자후,연구료DNA분자재불동pH화염농도조건하재입자표면적흡부화탈부행위。재pH<4.0적조건하,DNA통과소함적린산근기단여입자표면적Fe3+리자산생배위상호작용이흡부도입자표면,차과정수염농도영향불대。당pH>4.0시,유우용액중경양근리자적경쟁작용,사DNA적린산근기단불능여Fe3+리자배위,차시지능통과정전혹경건작용흡부,단시흡부효솔흔저。인차,Fe3+리자수식적금납미입자지재저pH조건하가이실현대DNA적고효흡부。저충기우배위작용적흡부효솔요고우염교작용,이차불회수도용액중염농도적영향。
Glutathione modified gold nanoparticles were charged with Fe3+,then the adsorption and desorption behaviors of DNA on the particle surface at different pH and different salt concentration were investigated. At pH<4. 0,DNA could be adsorbed efficiently on the particle surface driven by the coordination interactions be-tween the Fe3+ ions and phosphate groups of DNA,which was almost not affected by the salt concentration in the solutions. At pH>4. 0,the Fe3+ ions can’t coordinated with phosphate groups of DNA because of the com-petition of OH-ions and the adsorption was driven by electrostatic interaction or hydrogen bond,which showed low eficiency. Only at lower pH,DNA could be adsorbed efficiently on the particles surface driven by the coor-dination interactions which provided higher efficient adsorption than driven by the salt bridges,and was almost not affected by the salt concentration in the solutions.