CAJ | 학술논문

目的：研究在小鼠颌骨发育过程中长链非编码RNA(lncRNA)的表达谱变化情况。方法利用lncRNA-seq测序技术检测孕18 d及出生后14 d 的C57小鼠下颌骨组织样本中的lncRNA表达谱差异,经对原始数据进行预处理达到均一化后,筛选出差异表达lncRNA,进行分析。结果孕18 d与出生后14 d的C57小鼠下颌骨组织样本相比较,2倍以上变化并有显著差异(FDR≤0.001)的lncRNA,确定为差异表达lncRNA。2倍以上变化的共6617条,占所有lncRNA的17.16%；2倍以上升高的共3720条；2倍以上降低的共2897条；5倍以上升高的共714条；5倍以上降低的共288条；10倍以上升高为共645条；10倍以上降低的共211条。结论孕18 d与出生后14 d的C57小鼠下颌骨组织样本相比较,lncRNA表达谱发生显著变化。提示差异性表达的lncRNA可能参与了小鼠颌骨发育的调控。
목적：연구재소서합골발육과정중장련비편마RNA(lncRNA)적표체보변화정황。방법이용lncRNA-seq측서기술검측잉18 d급출생후14 d 적C57소서하합골조직양본중적lncRNA표체보차이,경대원시수거진행예처리체도균일화후,사선출차이표체lncRNA,진행분석。결과잉18 d여출생후14 d적C57소서하합골조직양본상비교,2배이상변화병유현저차이(FDR≤0.001)적lncRNA,학정위차이표체lncRNA。2배이상변화적공6617조,점소유lncRNA적17.16%；2배이상승고적공3720조；2배이상강저적공2897조；5배이상승고적공714조；5배이상강저적공288조；10배이상승고위공645조；10배이상강저적공211조。결론잉18 d여출생후14 d적C57소서하합골조직양본상비교,lncRNA표체보발생현저변화。제시차이성표체적lncRNA가능삼여료소서합골발육적조공。
Objective To analyze the expression of long non-coding RNA (lncRNA) in the jaw development process of mice. Methods lncRNA-seq sequencing technology was used to inspect the difference of lncRNA expression profile between C57 mice of embryonic day 18 and 2 weeks after birth. The lncRNAs with different expression levels were screened out after pretreatment and homogenization of raw data. The hierarchical clustering and volcano scatter diagram analysis were performed. Results Mandibular tissues of the C57 mice's of embryonic day 18 and 2 weeks after birth were compared. 6617 lncRNAs showed more than 2 times difference (FDR≤0.001) between the two groups were recognized as lncRNAs with differential expression, accounting for 17.16% of all lncRNAs. Among thses lncRNAs, 3720 increased more than 2 times and 2897 reduced more than 2 times, 714 increased more than 5 times and 288 reduced more than 5 times, 645 increased more than 10 times and 211 reduced more than 10 times. Conclusion In the jaw development process of mice, the lncRNA expression changes significantly, suggesting that lncRNAs may participate in the regulation of the jaw development.