CAJ | 학술논문

目的：建立基于实时荧光PCR技术的鸭源性成分的快速检测方法。方法：以鸭线粒体DNA序列为目的基因，设计并筛选了鸭源性特异性引物及探针，进行荧光定量PCR扩增，建立鸭源性成分检测方法。通过特异性、灵敏性、及盲样检测试验，对该体系进行验证。结果：该方法能够快速有效的检测鸭源性成分，具有较强的特异性及灵敏性，灵敏度约为0．01％（质量分数）；通过市售盲样肉制品的检测，表明该体系可用于定性检测加工肉制品中的鸭源性成分。结论：该方法特异性强，灵敏度高，可用于对肉制品中鸭源性成分的掺假鉴别。
목적：건립기우실시형광PCR기술적압원성성분적쾌속검측방법。방법：이압선립체DNA서렬위목적기인，설계병사선료압원성특이성인물급탐침，진행형광정량PCR확증，건립압원성성분검측방법。통과특이성、령민성、급맹양검측시험，대해체계진행험증。결과：해방법능구쾌속유효적검측압원성성분，구유교강적특이성급령민성，령민도약위0．01％（질량분수）；통과시수맹양육제품적검측，표명해체계가용우정성검측가공육제품중적압원성성분。결론：해방법특이성강，령민도고，가용우대육제품중압원성성분적참가감별。
Objective:A rapid detection method based on real-time PCR was established to detect the components of duck origin. Methods: The duck mitochondrial DNA was taken as target gene. The duck origin specific primers and probe were designed and screened to do real-time PCR amplification. And the detection method of components of duck origin was established. The system was verified by spe?cificity, sensitivity, and blind sample testing. Results: The method could fast and effectively detect the components of duck origin and had strong specificity and sensitivity. The sensitivity was about 0. 01%( mass fraction) . Through market blind detection of meat product, the system could be used for qualitative detection of duck origin components in processed meat products. Conclusion:the method with strong speci?ficity and high sensitivity could be used for identification of adulteration in meat duck origin components.