武警医学
武警醫學
무경의학
MEDICAL JOURNAL OF THE CHINESE PEOPLE'S ARMED POLICE FORCES
2015年
1期
11-12,15
,共3页
陈轶%陈益耀%蔡蔓妮%巫华志
陳軼%陳益耀%蔡蔓妮%巫華誌
진질%진익요%채만니%무화지
非酒精性脂肪性肝病%脂肪代谢%早期诊断%miR-122
非酒精性脂肪性肝病%脂肪代謝%早期診斷%miR-122
비주정성지방성간병%지방대사%조기진단%miR-122
NAFLD%fatty acid metabolism%early diagnosis%miR-122
目的：探讨microRNA-122（miR-122）在非酒精性脂肪肝病（non-alcoholic fatty liver disease，NAFLD）患者血清中的表达及其临床意义。方法采用荧光定量RT-PCR技术检测健康人、高脂血症患者、非酒精性单纯性脂肪肝（ non-alcoholic simply fatty liver，NAFL）及非酒精性脂肪性肝炎（non-alcoholic steatohepatitis，NASH）患者血清中miR-122的表达。结果各组miR-122的表达水平分别为5．94±1．21（2-ΔΔCt），5．99±1．03（2-ΔΔCt），8．67±0．72（2-ΔΔCt），10．55±0．83（2-ΔΔCt）。高脂血症患者和健康人血清中miR-122的表达量差异无统计学意义（P＞0．05）。与前两组相比，NAFL和NASH患者血清中miR-122的表达明显逐渐升高，差异有统计学意义（ P＜0．05）。结论非酒精性脂肪肝病患者血清中miR-122的表达量明显升高，提示miR-122参与了非酒精性脂肪肝的形成及进展过程，可作为诊断和评估组织学病变严重性的一种新的生物学指标，同时有望成为一个潜在的治疗靶点。
目的：探討microRNA-122（miR-122）在非酒精性脂肪肝病（non-alcoholic fatty liver disease，NAFLD）患者血清中的錶達及其臨床意義。方法採用熒光定量RT-PCR技術檢測健康人、高脂血癥患者、非酒精性單純性脂肪肝（ non-alcoholic simply fatty liver，NAFL）及非酒精性脂肪性肝炎（non-alcoholic steatohepatitis，NASH）患者血清中miR-122的錶達。結果各組miR-122的錶達水平分彆為5．94±1．21（2-ΔΔCt），5．99±1．03（2-ΔΔCt），8．67±0．72（2-ΔΔCt），10．55±0．83（2-ΔΔCt）。高脂血癥患者和健康人血清中miR-122的錶達量差異無統計學意義（P＞0．05）。與前兩組相比，NAFL和NASH患者血清中miR-122的錶達明顯逐漸升高，差異有統計學意義（ P＜0．05）。結論非酒精性脂肪肝病患者血清中miR-122的錶達量明顯升高，提示miR-122參與瞭非酒精性脂肪肝的形成及進展過程，可作為診斷和評估組織學病變嚴重性的一種新的生物學指標，同時有望成為一箇潛在的治療靶點。
목적：탐토microRNA-122（miR-122）재비주정성지방간병（non-alcoholic fatty liver disease，NAFLD）환자혈청중적표체급기림상의의。방법채용형광정량RT-PCR기술검측건강인、고지혈증환자、비주정성단순성지방간（ non-alcoholic simply fatty liver，NAFL）급비주정성지방성간염（non-alcoholic steatohepatitis，NASH）환자혈청중miR-122적표체。결과각조miR-122적표체수평분별위5．94±1．21（2-ΔΔCt），5．99±1．03（2-ΔΔCt），8．67±0．72（2-ΔΔCt），10．55±0．83（2-ΔΔCt）。고지혈증환자화건강인혈청중miR-122적표체량차이무통계학의의（P＞0．05）。여전량조상비，NAFL화NASH환자혈청중miR-122적표체명현축점승고，차이유통계학의의（ P＜0．05）。결론비주정성지방간병환자혈청중miR-122적표체량명현승고，제시miR-122삼여료비주정성지방간적형성급진전과정，가작위진단화평고조직학병변엄중성적일충신적생물학지표，동시유망성위일개잠재적치료파점。
Objective To analyze the expression levels of serum microRNA-122 ( miR-122) in non-alcoholic fatty liver dis-ease (NAFLD) patients, and its clinical significance.Methods Patients with normal liver, hyperlipidemia, non-alcoholic simply fat-ty liver(NAFL),non-alcoholic steatohepatitis(NASH)were included in this study.Blood samples of the patients were collected for a-nalysis.Real time quantitative PCR technique was used to measure the expression level of serum miR -122.Results The expression levels of serum miR-122 was 5.94 ±1.21(2 -ΔΔCt),5.99 ±1.03(2 -ΔΔCt),8.67 ±0.72(2-ΔΔCt)and 10.55 ±0.83(2 -ΔΔCt),respec-tively.The difference in the expression level between normal liver and hyperlipidemia patients was not statistically significant .Compa-ring with normal liver and hyperlipidemia patients , miR-122 presented higher expressions in NAFL patients (P<0.05),especially in NASH patients.Conclusion miR-122 plays an important role in NAFLD development and has early diagnostic value for indicating NAFLD.It may also be a potential therapeutic target in NAFLD with significant clinical value .
