中国医院用药评价与分析
中國醫院用藥評價與分析
중국의원용약평개여분석
EVALUATION AND ANAL YSIS OF DRUG-USE IN HOSPITALS OF CHINA
2015年
1期
47-49,50
,共4页
解玉波%王莉梅%姚铭%金向群
解玉波%王莉梅%姚銘%金嚮群
해옥파%왕리매%요명%금향군
清心滚痰胶囊%硫化汞%高效液相色谱法
清心滾痰膠囊%硫化汞%高效液相色譜法
청심곤담효낭%류화홍%고효액상색보법
Qingxin Guntan capsule%Mercuric sulfide%HPLC
目的:建立清心滚痰胶囊中硫化汞的含量测定方法。方法:采用高效液相色谱法。色谱柱为C18柱(4.6 mm ×250 mm,0.5μm),流动相为甲醇-0.01 mol/L磷酸氢二钠缓冲液(含0.01%二乙基二硫代氨基甲酸钠)(V∶V=73∶27,用磷酸调节pH至7.5),检测波长为270 nm,流速为0.8 ml/min,柱温为30℃。结果:Hg2+进样量在0.4~2.0μg 范围内线性关系良好( r =0.99997),平均回收率为93.83%,RSD为1.94%(n=6)。结论:该法简便、准确、专属性强,可用于清心滚痰胶囊中硫化汞的含量测定。
目的:建立清心滾痰膠囊中硫化汞的含量測定方法。方法:採用高效液相色譜法。色譜柱為C18柱(4.6 mm ×250 mm,0.5μm),流動相為甲醇-0.01 mol/L燐痠氫二鈉緩遲液(含0.01%二乙基二硫代氨基甲痠鈉)(V∶V=73∶27,用燐痠調節pH至7.5),檢測波長為270 nm,流速為0.8 ml/min,柱溫為30℃。結果:Hg2+進樣量在0.4~2.0μg 範圍內線性關繫良好( r =0.99997),平均迴收率為93.83%,RSD為1.94%(n=6)。結論:該法簡便、準確、專屬性彊,可用于清心滾痰膠囊中硫化汞的含量測定。
목적:건립청심곤담효낭중류화홍적함량측정방법。방법:채용고효액상색보법。색보주위C18주(4.6 mm ×250 mm,0.5μm),류동상위갑순-0.01 mol/L린산경이납완충액(함0.01%이을기이류대안기갑산납)(V∶V=73∶27,용린산조절pH지7.5),검측파장위270 nm,류속위0.8 ml/min,주온위30℃。결과:Hg2+진양량재0.4~2.0μg 범위내선성관계량호( r =0.99997),평균회수솔위93.83%,RSD위1.94%(n=6)。결론:해법간편、준학、전속성강,가용우청심곤담효낭중류화홍적함량측정。
OBJECTIVE:To develop a method for determination of mercuric sulfide in Qingxin Guntan capsule . METHODS:The determination of mercuric sulfide in Qingxin Guntan capsule was carried out by HPLC using C 18 (4.6 mm ×250 mm,0.5 μm) chromatographic column with the mobile phase consisting of methanol-0.01 mol/L disodium phosphate buffer ( containing 0.01% sodium diethyldithiocarbamate ) ( V∶V=73∶27 , with pH adjusted to 7.5 using phosphoric acid ) at the detection wavelength of 270 nm,flow rate of 0.8 ml/min and the column temperature of 30 ℃.RESULTS:The linear range of Hg 2+was 0.40-2.00 μg( r=0.999 97) with an average recovery of 93.83%and RSD of 1.94%( n =6 ) . CONCLUSIONS:The method is simple and specific and it can be applied for determination of mercuric sulfide in Qingxin Guntan capsule .