CAJ | 학술논문

目的：研究不同剂量氨氯地平抑制小鼠黑色素瘤高转移细胞株B16转移的作用，并探讨其作用机制。方法：选取64只C57BL／6J小鼠，将黑色素瘤髙转移细胞株B16接种在小鼠腹股沟皮下（2×106个／只）。将接种后的小鼠以随机数字表法分为对照组和氨氯地平高、中、低剂量治疗组，对照组小鼠仅给予0.9％氯化钠注射液；治疗组小鼠给予不同剂量的氨氯地平治疗，分别为1 mg／kg（低剂量组）、3 mg／kg（中剂量组）和10 mg／kg（高剂量组）。观察各组小鼠肿瘤生长情况及身体情况；观察各组小鼠肿瘤肺转移情况，计算肺转移的结节及肿瘤抑瘤率；采用免疫组织化学法检测各组小鼠肿瘤组织中白细胞介素8（IL-8）蛋白表达水平；采用逆转录-聚合酶链反应（RT-PCR）检测各组小鼠肿瘤组织中IL-8的信使核糖核酸（mRNA）表达水平。结果：氨氯地平对小鼠黑色素瘤高转移细胞株B16的自发性肺转移有显著的抑制作用；治疗组小鼠肿瘤组织中IL-8蛋白表达均明显减弱，且随氨氯地平剂量的增加而减弱；治疗组小鼠肿瘤组织中IL-8的mRNA的表达明显低于对照组，且IL-8的mRNA的表达随氨氯地平剂量的增加而减弱。结论：氨氯地平对小鼠黑色素瘤高转移细胞株B16有一定的抑瘤和抑制肺转移作用，其作用可能与影响IL-8的表达有关。
목적：연구불동제량안록지평억제소서흑색소류고전이세포주B16전이적작용，병탐토기작용궤제。방법：선취64지C57BL／6J소서，장흑색소류고전이세포주B16접충재소서복고구피하（2×106개／지）。장접충후적소서이수궤수자표법분위대조조화안록지평고、중、저제량치료조，대조조소서부급여0.9％록화납주사액；치료조소서급여불동제량적안록지평치료，분별위1 mg／kg（저제량조）、3 mg／kg（중제량조）화10 mg／kg（고제량조）。관찰각조소서종류생장정황급신체정황；관찰각조소서종류폐전이정황，계산폐전이적결절급종류억류솔；채용면역조직화학법검측각조소서종류조직중백세포개소8（IL-8）단백표체수평；채용역전록-취합매련반응（RT-PCR）검측각조소서종류조직중IL-8적신사핵당핵산（mRNA）표체수평。결과：안록지평대소서흑색소류고전이세포주B16적자발성폐전이유현저적억제작용；치료조소서종류조직중IL-8단백표체균명현감약，차수안록지평제량적증가이감약；치료조소서종류조직중IL-8적mRNA적표체명현저우대조조，차IL-8적mRNA적표체수안록지평제량적증가이감약。결론：안록지평대소서흑색소류고전이세포주B16유일정적억류화억제폐전이작용，기작용가능여영향IL-8적표체유관。
OBJECTIVE:To study the mechanism underlying the inhibitory effect of amlodipine on melanoma cell line B16.METHODS: The groin region in a total of 64 C57BL/6J mice was inoculated subcutaneously with highly metastatic melanoma cell line B16 (2 ×106 cells/rats) then the mice were randomized to receive either 0.9%sodium chloride injection(control group) or amlodipine [1 mg/kg(low dose group), 3 mg/kg(medium dose group) or 10 mg/kg ( high dose group ) ] .The tumor growth and physical conditions of mice were followed; pulmonary metastases was followed with nodules in pulmonary metastasis and tumor inhibition rate calculated ;immunohistochemistry was employed to detect the level of interleukin-8(IL-8) protein in tumor tissue, and reverse transcription-polymerase chain reaction (RT-PCR) was employed to detect the level of mRNA in IL-8 in tumor tissue.RESULTS: Amlodipine exhibited significant inhibitory effect on the spontaneous pulmonary metastases of the highly metastatic melanoma cell line B 16 in mice;the expression of IL-8 protein in the treatment group significantly decreased in dose-dependent manner; mRNA expression of IL-8 was significantly lower in the treatment groups than in the control group , and the mRNA expression of IL-8 decreased with the increase of the doses of amlodipine .CONCLUSIONS: The inhibitory effect of amlodipine on highly metastatic melanoma cell line B 16 in mice might be associated with its impact on the expression of IL-8.