中国医院用药评价与分析
中國醫院用藥評價與分析
중국의원용약평개여분석
EVALUATION AND ANAL YSIS OF DRUG-USE IN HOSPITALS OF CHINA
2015年
1期
27-30
,共4页
张伟%张鹤凤%刘静静%康艳生%王晓琴%戴贵东
張偉%張鶴鳳%劉靜靜%康豔生%王曉琴%戴貴東
장위%장학봉%류정정%강염생%왕효금%대귀동
氧化苦参碱%异丙肾上腺素%心脏重构%抗氧化酶%一氧化氮
氧化苦參堿%異丙腎上腺素%心髒重構%抗氧化酶%一氧化氮
양화고삼감%이병신상선소%심장중구%항양화매%일양화담
Oxymatrine%Cardiac remodeling%Isoproterenol%Antioxidase%NO
目的：研究氧化苦参碱对异丙肾上腺素致心肌肥厚大鼠心脏重构的保护作用，并探讨其作用机制。方法：给予Sprague-dawley大鼠连续7 d皮下注射异丙肾上腺素5 mg／（ kg· d），以建立大鼠心肌肥厚模型，观察氧化苦参碱对模型大鼠体质量，心肌肥厚指数，心肌组织形态学改变，超氧化物歧化酶、丙二醛及血清一氧化氮含量，一氧化氮合酶活性的影响。结果：氧化苦参碱可明显降低模型大鼠心肌肥厚指数、心肌细胞直径与横截面积、心肌间质胶原纤维含量；增强了血清及心肌组织中超氧化物歧化酶的活力，并相应地降低了脂质过氧化物丙二醛的含量；增强了血清中总一氧化氮合酶的活性，增加了血清一氧化氮的含量，但降低了诱导型一氧化氮合酶的活性。结论：氧化苦参碱对异丙肾上腺素致心肌肥厚大鼠有明显的心脏重构保护作用，其作用机制可能与提高机体抗氧化酶活性和调节血清一氧化氮含量／一氧化氮合酶活性有关。
目的：研究氧化苦參堿對異丙腎上腺素緻心肌肥厚大鼠心髒重構的保護作用，併探討其作用機製。方法：給予Sprague-dawley大鼠連續7 d皮下註射異丙腎上腺素5 mg／（ kg· d），以建立大鼠心肌肥厚模型，觀察氧化苦參堿對模型大鼠體質量，心肌肥厚指數，心肌組織形態學改變，超氧化物歧化酶、丙二醛及血清一氧化氮含量，一氧化氮閤酶活性的影響。結果：氧化苦參堿可明顯降低模型大鼠心肌肥厚指數、心肌細胞直徑與橫截麵積、心肌間質膠原纖維含量；增彊瞭血清及心肌組織中超氧化物歧化酶的活力，併相應地降低瞭脂質過氧化物丙二醛的含量；增彊瞭血清中總一氧化氮閤酶的活性，增加瞭血清一氧化氮的含量，但降低瞭誘導型一氧化氮閤酶的活性。結論：氧化苦參堿對異丙腎上腺素緻心肌肥厚大鼠有明顯的心髒重構保護作用，其作用機製可能與提高機體抗氧化酶活性和調節血清一氧化氮含量／一氧化氮閤酶活性有關。
목적：연구양화고삼감대이병신상선소치심기비후대서심장중구적보호작용，병탐토기작용궤제。방법：급여Sprague-dawley대서련속7 d피하주사이병신상선소5 mg／（ kg· d），이건립대서심기비후모형，관찰양화고삼감대모형대서체질량，심기비후지수，심기조직형태학개변，초양화물기화매、병이철급혈청일양화담함량，일양화담합매활성적영향。결과：양화고삼감가명현강저모형대서심기비후지수、심기세포직경여횡절면적、심기간질효원섬유함량；증강료혈청급심기조직중초양화물기화매적활력，병상응지강저료지질과양화물병이철적함량；증강료혈청중총일양화담합매적활성，증가료혈청일양화담적함량，단강저료유도형일양화담합매적활성。결론：양화고삼감대이병신상선소치심기비후대서유명현적심장중구보호작용，기작용궤제가능여제고궤체항양화매활성화조절혈청일양화담함량／일양화담합매활성유관。
OBJECTIVE:To investigate the protective effect of oxymatrine on cardiac remodeling in rats with isoproterenol ( ISO )-induced heart failure and explore its action mechanism .METHODS: Myocardial hypertrophy model was established in Sprague-Dawley rats by subcutaneous injection with isoproterenol [ISO, 5 mg/(kg· d)] for seven days .The body weight , myocardial hypertrophy indexes and histomorphology were followed to evaluate the effect of oxymatrine on cardiac remodeling in rats with isoproterenol-induced myocardial hypertrophy .In addition , levels of superoxide dismutase(SOD), malondialdehyde and serum nitric oxide(NO) and the activity of NO synthase(NOS) in serum were measured .RESULTS: Oxymatine treatment significantly decreased the myocaridial hypertrophy indexes , cardiomyocyte diameter and the content of cardiac interstitial collagen fiber , enhanced the activity of superoxide dismutase ( SOD ) in both serum and cardiac tissue and accordingly decreased the content of lipid peroxide malondialdehyde , enhanced the activity of serum NOS and serum content of NO but decreased the activity of Nitric-Oxide Synthase of the induced type .CONCLUTIONS: The mechanisms underlying the significant protective effect of oxymatine against cardiac remodeling might be attributed to its action in enhancing the activity of antioxidase and regulating NO content and NOS activity .
