烟草科技
煙草科技
연초과기
TOBACCO SCIENCE & TECHNOLOGY
2015年
1期
7-11
,共5页
杨健%梁太波%李海江%尹启生%张艳玲%周汉平%张仕祥
楊健%樑太波%李海江%尹啟生%張豔玲%週漢平%張仕祥
양건%량태파%리해강%윤계생%장염령%주한평%장사상
纳米碳溶胶%烤烟%根系%生理特性%钾吸收
納米碳溶膠%烤煙%根繫%生理特性%鉀吸收
납미탄용효%고연%근계%생리특성%갑흡수
Nano-carbon sol%Flue-cured tobacco%Root system%Physiological characteristic%Potassium absorption
为明确纳米碳溶胶促进烤烟钾吸收的生理基础,利用水培试验和无菌培养试验,研究了不同浓度的纳米碳溶胶对两个烤烟品种根系生理特性、钾素吸收及其相关基因表达量的影响。结果表明:当纳米碳溶胶浓度(质量浓度)分别为10~20和5~10 mg/L时,能显著促进K326和红花大金元(红大)根系生长,增加根系和地上部生物量,提高烟叶钾素吸收及积累量。随着纳米碳溶胶浓度的升高,两个烤烟品种的根系活力、根系吸收面积、根系阳离子交换量和根系可溶性蛋白含量呈现先增加后降低的趋势。K326和红大K+通道基因NKT1和转运体基因NtHAK1的表达量显著增加,提高了根系对钾的吸收能力。
為明確納米碳溶膠促進烤煙鉀吸收的生理基礎,利用水培試驗和無菌培養試驗,研究瞭不同濃度的納米碳溶膠對兩箇烤煙品種根繫生理特性、鉀素吸收及其相關基因錶達量的影響。結果錶明:噹納米碳溶膠濃度(質量濃度)分彆為10~20和5~10 mg/L時,能顯著促進K326和紅花大金元(紅大)根繫生長,增加根繫和地上部生物量,提高煙葉鉀素吸收及積纍量。隨著納米碳溶膠濃度的升高,兩箇烤煙品種的根繫活力、根繫吸收麵積、根繫暘離子交換量和根繫可溶性蛋白含量呈現先增加後降低的趨勢。K326和紅大K+通道基因NKT1和轉運體基因NtHAK1的錶達量顯著增加,提高瞭根繫對鉀的吸收能力。
위명학납미탄용효촉진고연갑흡수적생리기출,이용수배시험화무균배양시험,연구료불동농도적납미탄용효대량개고연품충근계생리특성、갑소흡수급기상관기인표체량적영향。결과표명:당납미탄용효농도(질량농도)분별위10~20화5~10 mg/L시,능현저촉진K326화홍화대금원(홍대)근계생장,증가근계화지상부생물량,제고연협갑소흡수급적루량。수착납미탄용효농도적승고,량개고연품충적근계활력、근계흡수면적、근계양리자교환량화근계가용성단백함량정현선증가후강저적추세。K326화홍대K+통도기인NKT1화전운체기인NtHAK1적표체량현저증가,제고료근계대갑적흡수능력。
In order to reveal the physiological basis of potassium absorption promoted by nano-carbon sol, a water-culture experiment and an enclosed sterile culture experiment were carried out. The effects of nano-carbon sol application on the physiological characteristics of root system, potassium absorption and related gene expression level of flue-cured tobacco cv. K326 and cv. Hongda were studied. The results showed that at the concentrations of 10-20 and 5-10 mg/L, nano-carbon sol significantly promoted the growth of root system, increased the shoot and root biomass and improved potassium absorption and accumulation in leaves of K326 and Hongda. With the increase of nano-carbon sol concentration, the vigor, absorbing area, cation exchange capacity and soluble protein content of root system of K326 and Hongda increased first then decreased. The expression levels of K+ channel gene NKT1 and K+ transporter gene NtHAK1 in K326 and Hongda increased significantly, which promoted the potassium absorbing ability of root system.