CAJ | 학술논문

以陆地棉抗病品种中棉所12和感病品种新陆早7号为材料,利用 Solexa 高通量测序技术调查枯萎病菌诱导后不同时间感、抗陆地棉品种转录因子家族及转录因子的表达情况。结果表明,枯萎病菌诱导后,抗病品种中棉所12有39个转录因子家族的433个转录因子在至少一个比对组中表达活性发生变化；感病品种新陆早7号则有52个转录因子家族的588个转录因子在至少一个比对组中表达活性发生变化。新陆早7号对枯萎病菌响应的转录因子及转录因子家族的数目明显多于中棉所12,且2个品种的下调基因数目均多于上调基因。随着枯萎病菌诱导后时间延长,两品种对枯萎病菌诱导响应的转录因子家族及转录因子数量均呈现先增加后降低的变化趋势,中棉所12在枯萎病菌诱导后6 h 达最多,而新陆早7号在诱导后3 h 达最多。在6个比对组中,表达活性均发生变化的重叠转录因子,中棉所12中有9个,隶属于6个转录因子家族；新陆早7号中有31个,隶属于17个转录因子家族。不同抗病性品种对枯萎病的响应有较强的品种特异性,除37个共有的转录因子家族外,2个转录因子家族是中棉所12所特有,15个转录因子家族是新陆早7号所特有。
이륙지면항병품충중면소12화감병품충신륙조7호위재료,이용 Solexa 고통량측서기술조사고위병균유도후불동시간감、항륙지면품충전록인자가족급전록인자적표체정황。결과표명,고위병균유도후,항병품충중면소12유39개전록인자가족적433개전록인자재지소일개비대조중표체활성발생변화；감병품충신륙조7호칙유52개전록인자가족적588개전록인자재지소일개비대조중표체활성발생변화。신륙조7호대고위병균향응적전록인자급전록인자가족적수목명현다우중면소12,차2개품충적하조기인수목균다우상조기인。수착고위병균유도후시간연장,량품충대고위병균유도향응적전록인자가족급전록인자수량균정현선증가후강저적변화추세,중면소12재고위병균유도후6 h 체최다,이신륙조7호재유도후3 h 체최다。재6개비대조중,표체활성균발생변화적중첩전록인자,중면소12중유9개,대속우6개전록인자가족；신륙조7호중유31개,대속우17개전록인자가족。불동항병성품충대고위병적향응유교강적품충특이성,제37개공유적전록인자가족외,2개전록인자가족시중면소12소특유,15개전록인자가족시신륙조7호소특유。
The Solexa sequencing technique was used to study the expression changes of transcription factors and their families in upland cotton cultivars susceptible and resistant to Fusarium wilt. A total of 39 transcription factor families and 433 transcription factors in Zhongmiansuo 12 and 52 transcription factor families and 588 transcription factors in Xinluzao 7 were changed in ex-pression in at least one comparison group induced by Fusarium wilt. The number of transcription factor families responsed to Fusarium wilt was more in Xinluzao 7 than in Zhongmiansuo 12, and the number of down-regulated genes was more than that of up-regulated genes in two varieties. After the induction by Fusarium wilt, in the process of time, the number of transcription factor families and transcription factors responsed to the Fusarium wilt in two cultivars showed the same changing trend of increase first and decrease then. The maximum number was reached after six hours in Zhongmiansuo 12, while at three hours after the inducing in Xinluzao 7. Among six comparison groups, there were nine overlapping transcription factors in six transcription factor families of Zhongmiansuo 12 and 31 overlapping transcription factors in 17 transcription factor families of Xinluzao 7. The expression of transcription factor showed strong cultivar specificity among varieties tolerant to Fusarium wilt. Except for 37 transcription factor families shared between two cultivars, two transcription factor families were unique to Zhongmiansuo 12 and Zhongmiansuo 15 transcription factor families were unique to Xinluzao 7.