CAJ | 학술논문

目的：：研究Crnn蛋白在食管鳞癌组织( ESCC)中的表达及其在ESCC发生、发展及预后中的作用。方法：采用免疫组织化学方法检测组织阵列仪取样的100例ESCC组织(观察组)和相应正常食管组织(对照组)中Crnn蛋白的表达情况，并探讨其与ESCC患者年龄、性别、肿瘤细胞分化程度、淋巴结转移、TNM分期、大体分型和5年生存率的关系。结果：ESCC组织中Crnn蛋白表达阳性率(45.6%)明显低于正常组织中的表达阳性率(93.7%)(P<0.05)；肿瘤组织Crnn表达下调率为53.2%；Crnn基因表达与淋巴结转移、TNM分期有关，而与年龄、性别、肿瘤细胞分化程度、和大体分型无关；Crnn蛋白表达下调的食管鳞癌患者的5年生存率明显低于Crnn蛋白正常表达的食管鳞癌患者。结论： Crnn基因表达下调可能会促进食管鳞癌的发生、发展，是食管鳞癌组织中候选的抑癌基因。
목적：：연구Crnn단백재식관린암조직( ESCC)중적표체급기재ESCC발생、발전급예후중적작용。방법：채용면역조직화학방법검측조직진렬의취양적100례ESCC조직(관찰조)화상응정상식관조직(대조조)중Crnn단백적표체정황，병탐토기여ESCC환자년령、성별、종류세포분화정도、림파결전이、TNM분기、대체분형화5년생존솔적관계。결과：ESCC조직중Crnn단백표체양성솔(45.6%)명현저우정상조직중적표체양성솔(93.7%)(P<0.05)；종류조직Crnn표체하조솔위53.2%；Crnn기인표체여림파결전이、TNM분기유관，이여년령、성별、종류세포분화정도、화대체분형무관；Crnn단백표체하조적식관린암환자적5년생존솔명현저우Crnn단백정상표체적식관린암환자。결론： Crnn기인표체하조가능회촉진식관린암적발생、발전，시식관린암조직중후선적억암기인。
Objective: To investigate the expression of Crnn in human esophageal squamous cell carcinoma( ESCC) and the role of Crnn in the occurrence, development and prognosis of ESCC. Methods:The expression levels of Crnn in 100 ESCC tissues ( observation group ) were compared with that in corresponding normal esophageal tissues( control group) by immunohistochemical tech-niques. Its correlation with age, gender, tumor-cell differentiation, lymph-node metastasis, TNM stage, general classification and five-year survival rate of ESCC patients were analyzed. Results:The expression rate of Crnn in the observation group was 45 . 6%, which was obviously lower than the control group (93. 7%), the down-regulation rate of Crnn in tumor tissues was 53. 2%, the expression of Crnn gene was correlated with lymph nodes metastasis and TMN stage, but not with age, gender, tumor-cell differentiation and general classification. The five-year survival rate of ES-CC patients with the down expression of Crnn gene was lower than ESCC patients with normal ex-pression of Crnn gene. Conclusion: The down expression of Crnn gene may promote the occur-rence and development of ESCC. Crnn was the candidate tumor suppressor gene of ESCC.