CAJ | 학술논문

目的：探讨 PCR-反向点杂交基因分型检测阳性率与病毒核酸载量间的关系。方法采用 PCR 荧光法对1162例女性患者进行 HR-HPV DNA 载量检测，采用 PCR-反向点杂交法对其中141例高危 HR-HPV DNA 阳性标本进行 HPV 基因分型检测。结果基因分型检测总阳性率为68.8％，基因分型检测阳性率与 HR-HPV DNA 载量对数值呈显著正相关（r＝0.944，P﹤0.01），其二次项曲线拟合公式为 Y＝-1.806＋0.558X-0.031X2（Y 为基因分型阳性率，X 为 HR-HPV DNA 载量对数值）。不同载量组间的基因分型检测阳性率差异具有统计学意义（P﹤0.01）：当 HR-HPV DNA 载量在104~105拷贝/ ml、105~106拷贝/ ml、106~107拷贝/ ml 和＞107拷贝/ ml 时，使用不同厂家基因分型检测试剂其阳性率分别为27.8％、48.5％、74.0％、97.5％和33.3％、51.5％、78.0％、97.5％。结论PCR-反向点杂交法基因分型检测阳性率与其核酸载量相关。
목적：탐토 PCR-반향점잡교기인분형검측양성솔여병독핵산재량간적관계。방법채용 PCR 형광법대1162례녀성환자진행 HR-HPV DNA 재량검측，채용 PCR-반향점잡교법대기중141례고위 HR-HPV DNA 양성표본진행 HPV 기인분형검측。결과기인분형검측총양성솔위68.8％，기인분형검측양성솔여 HR-HPV DNA 재량대수치정현저정상관（r＝0.944，P﹤0.01），기이차항곡선의합공식위 Y＝-1.806＋0.558X-0.031X2（Y 위기인분형양성솔，X 위 HR-HPV DNA 재량대수치）。불동재량조간적기인분형검측양성솔차이구유통계학의의（P﹤0.01）：당 HR-HPV DNA 재량재104~105고패/ ml、105~106고패/ ml、106~107고패/ ml 화＞107고패/ ml 시，사용불동엄가기인분형검측시제기양성솔분별위27.8％、48.5％、74.0％、97.5％화33.3％、51.5％、78.0％、97.5％。결론PCR-반향점잡교법기인분형검측양성솔여기핵산재량상관。
Objective To investigate the correlation between the positive rate of PCR-reverse dot blot genotyping test and the loads of the viral nucleic acid. Methods The fluorescent PCR assay was used to detect the high-risk HPV(HR-HPV)DNA loads in the cervical mucus samples from 1162 female pa-tients. Patients with positive HR-HPV DNA were further analyzed by PCR-reverse dot blot hybridization as-say for HPV genotyping. Results The overall positive rate of genotyping test was 68. 8% . The positive rate of genotyping test had a significant positive correlation with the Log Koc of HR-HPV DNA loads(r=0. 944, P﹤0. 01). The quadratic curve fitting formula was Y= -1. 806+0. 558X-0. 031X2(Y for genotyping positive rate,X for Log Koc of HR-HPV DNA loads). There were significant differences with the positive rate of gen-otyping test among patients with different viral loads(P﹤0. 01). When HR-HPV DNA loads were 104-105 copies/ ml,105-106 copies/ ml,106-107 copies/ ml and more than 107 copies/ ml,the positive rate of HPV genotyping test were 27. 8% ,48. 5% ,74. 0% ,97. 5% and 33. 3% ,51. 5% ,78. 0% ,97. 5% respective-ly by using different genotyping detection reagents. Conclusion The positive rate of PCR-reverse dot blot genotyping test was correlated with the loads of HPV nucleic acid.