CAJ | 학술논문

目的：检测泌尿生殖道沙眼衣原体感染者血清中抗重组衣原体质粒编码蛋白3（rPgp3）、衣原体蛋白酶样活性因子（ rCPAF）、Ct143编码蛋白（ rCT143）、Ct101编码蛋白（ rCT101）、Ct694编码蛋白（ rCT694）、Ct875编码蛋白（ rCT875）、Ct813编码蛋白（ rCT813）、主要外膜蛋白（rMOMP）、膜蛋白 A（rIncA）和热休克蛋白60（rHsp60）的 IgG 抗体，比较上述几种蛋白的抗原性。方法将编码上述10种蛋白的基因重组质粒转化入大肠杆菌，经 IPTG 诱导表达并鉴定后，用裂解液包被谷胱甘肽预包被的 ELISA 孔板，饱和板底的谷胱甘肽。收集天津医科大学总医院性病门诊经胶体金法和单克隆抗体免疫荧光法检测沙眼衣原体两者均阳性者的血清50份和两者均阴性者的血清10份，进行 ELISA 法检测。结果50份沙眼衣原体感染者血清中 rPgp3、rCPAF、rCT143、rCT101、rCT694、rCT875、rCT813、rMOMP、rIncA、rHsp60蛋白抗体的检出率分别是44例（88％）、38例（76％）、37例（74％）、36例（72％）、33例（66％）、31例（62％）、30例（60％）、26例（52％）、24例（48％）、17例（34％）。10份未感染者血清未检测到上述蛋白的抗体。结论上述10种沙眼衣原体抗原蛋白中，rPgp3抗原性最强，抗体检出率最高，其次是 rCPAF 和 rCT143；rHsp60抗原性最弱，抗体检出率最低。
목적：검측비뇨생식도사안의원체감염자혈청중항중조의원체질립편마단백3（rPgp3）、의원체단백매양활성인자（ rCPAF）、Ct143편마단백（ rCT143）、Ct101편마단백（ rCT101）、Ct694편마단백（ rCT694）、Ct875편마단백（ rCT875）、Ct813편마단백（ rCT813）、주요외막단백（rMOMP）、막단백 A（rIncA）화열휴극단백60（rHsp60）적 IgG 항체，비교상술궤충단백적항원성。방법장편마상술10충단백적기인중조질립전화입대장간균，경 IPTG 유도표체병감정후，용렬해액포피곡광감태예포피적 ELISA 공판，포화판저적곡광감태。수집천진의과대학총의원성병문진경효체금법화단극륭항체면역형광법검측사안의원체량자균양성자적혈청50빈화량자균음성자적혈청10빈，진행 ELISA 법검측。결과50빈사안의원체감염자혈청중 rPgp3、rCPAF、rCT143、rCT101、rCT694、rCT875、rCT813、rMOMP、rIncA、rHsp60단백항체적검출솔분별시44례（88％）、38례（76％）、37례（74％）、36례（72％）、33례（66％）、31례（62％）、30례（60％）、26례（52％）、24례（48％）、17례（34％）。10빈미감염자혈청미검측도상술단백적항체。결론상술10충사안의원체항원단백중，rPgp3항원성최강，항체검출솔최고，기차시 rCPAF 화 rCT143；rHsp60항원성최약，항체검출솔최저。
Objective To detect the antibodies against recombinant chlamydial plasmid-encoded protein 3(rPgp3),chlamydial protease-like activity factor(rCPAF),Ct143 encoded protein(rCT143), Ct101 encoded protein(rCT101),Ct694 encoded protein(rCT694),Ct813 encoded protein(rCT813), Chlamydia membrane protein A(rIncA),Ct875 encoded protein(rCT875),major outer membrane protein (rMOMP)and heat shock protein 60( rHsp60)in serum samples collected form patients with urogenital Chlamydia trachomatis(Ct)infection and to evaluate the antigenicity of those proteins. Methods The re-combinant plasmids expressing the 10 proteins and a blank plasmid were transformed into E. coli BL21 strains,respectively. The transformed E. coli BL21 strains were induced by isopropyl β-D-1-thiogalactopyr-anoside(IPTG)to express recombinant proteins. The glutathione pre-coated 96-well ELISA plates were coa-ted with lysates. Serum samples were collected from 50 patients with Ct infection and 10 patients without Ct infection. ELISA was performed to detect the antibodies against 10 recombinant proteins. Results The anti-bodies against rPgp3,rCPAF,rCT143,rCT101,rCT694,rCT875,rCT813,rMOMP,rIncA and rHsp60 proteins were respectively detected in 44 cases(88% ),38 cases(76% ),37 cases(74% ),36 cases (72% ),33 cases(66% ),31 cases(62% ),30 cases(60% ),26 cases(52% ),24 cases(48% )and 17 cases(34% )out of 50 serum samples. No antibodies against 10 recombinant proteins were detected in the serum samples collected from patients without Ct infection. Conclusion The rPgp3 protein showed the strongest antigenicity among all of the studied proteins,followed by rCPAF and rCT143 proteins. The rHsp60 protein showed the lowest antigenicity.