基层医学论坛
基層醫學論罈
기층의학론단
PUBLIC MEDICAL FORUM MAGAZINE
2015年
4期
435-438
,共4页
方海林%李军孝%姚林明%赵涛
方海林%李軍孝%姚林明%趙濤
방해림%리군효%요림명%조도
松果菊苷%成骨细胞%细胞增殖%MAPK/ERK%BMP/Smad%ERK/BMP-2
鬆果菊苷%成骨細胞%細胞增殖%MAPK/ERK%BMP/Smad%ERK/BMP-2
송과국감%성골세포%세포증식%MAPK/ERK%BMP/Smad%ERK/BMP-2
Echinacoside%Osteoblast%Cell proliferation%MAPK/ERK%BMP/Smad%ERK/BMP-2
目的:考察松果菊苷对大鼠成骨细胞增殖的影响及作用机制。方法将不同浓度的松果菊苷(0.01,0.1,1.0,10.0,100 nmol/L)及MAPK/ERK信号通路抑制剂PD98059(20μmol/L)与成骨细胞共培养12,24,36,48,60 h后,采用MTT法检测细胞增殖情况。酶联免疫吸附法(ELISA)和qRT-PCR法检测细胞上清中和成骨细胞中骨钙素(BGP)和骨形态发生蛋白(BMP-2)的表达水平。Western blotting检测成骨细胞中Smad4、ERK1/2和p-ERK1/2的蛋白表达水平。结果松果菊苷能够促进大鼠成骨细胞的增殖,而且能诱导ERK 的磷酸化从而激活 MAPK/ERK 信号通路,并提高成骨细胞中 BMP-2和 Smad4的表达而激活BMP/Smad信号通路。加入PD98059后,松果菊苷诱导的BMP-2和Smad4表达及成骨细胞增殖均受到抑制。结论松果菊苷可通过激活ERK/BMP-2信号通路促进大鼠的成骨细胞增殖。
目的:攷察鬆果菊苷對大鼠成骨細胞增殖的影響及作用機製。方法將不同濃度的鬆果菊苷(0.01,0.1,1.0,10.0,100 nmol/L)及MAPK/ERK信號通路抑製劑PD98059(20μmol/L)與成骨細胞共培養12,24,36,48,60 h後,採用MTT法檢測細胞增殖情況。酶聯免疫吸附法(ELISA)和qRT-PCR法檢測細胞上清中和成骨細胞中骨鈣素(BGP)和骨形態髮生蛋白(BMP-2)的錶達水平。Western blotting檢測成骨細胞中Smad4、ERK1/2和p-ERK1/2的蛋白錶達水平。結果鬆果菊苷能夠促進大鼠成骨細胞的增殖,而且能誘導ERK 的燐痠化從而激活 MAPK/ERK 信號通路,併提高成骨細胞中 BMP-2和 Smad4的錶達而激活BMP/Smad信號通路。加入PD98059後,鬆果菊苷誘導的BMP-2和Smad4錶達及成骨細胞增殖均受到抑製。結論鬆果菊苷可通過激活ERK/BMP-2信號通路促進大鼠的成骨細胞增殖。
목적:고찰송과국감대대서성골세포증식적영향급작용궤제。방법장불동농도적송과국감(0.01,0.1,1.0,10.0,100 nmol/L)급MAPK/ERK신호통로억제제PD98059(20μmol/L)여성골세포공배양12,24,36,48,60 h후,채용MTT법검측세포증식정황。매련면역흡부법(ELISA)화qRT-PCR법검측세포상청중화성골세포중골개소(BGP)화골형태발생단백(BMP-2)적표체수평。Western blotting검측성골세포중Smad4、ERK1/2화p-ERK1/2적단백표체수평。결과송과국감능구촉진대서성골세포적증식,이차능유도ERK 적린산화종이격활 MAPK/ERK 신호통로,병제고성골세포중 BMP-2화 Smad4적표체이격활BMP/Smad신호통로。가입PD98059후,송과국감유도적BMP-2화Smad4표체급성골세포증식균수도억제。결론송과국감가통과격활ERK/BMP-2신호통로촉진대서적성골세포증식。
ObjectiveTo investigate the effect of echinacoside(ECH)on cell proliferation of rat osteoblast and its mechanism.MethodThe osteoblast was incubated with different concentration of ECH(0.01、0.1、1.0、10.0、100 nmol/L)or PD98059(20 μmol/L)which is an inhibitor of MAPK/ERK for 12、24、36、48 or 60 h. MTT method was used for the detection of cell proliferation. The protein and mRNA level of BGP and BMP-2 in supernatant and cells was measured by ELISA and qRT-PCR. Expression of smad4、ERK1/2 and p-ERK1/2 was detected by western blotting. ResultsThe cell proliferation of osteoblast was promoted by ECH. ECH induced expression of BMP-2 and smad4 to activate BMP/smad pathway,and also promoted the phosphorylation of ERK1/2 to activate MAPK/ERK pathway. While the activation of BMP/smad pathway was inhibited by PD98059.ConclusionsEchinacoside promotes cell proliferation of rat osteoblast through activating of ERK/BMP-2 signaling pathway.