临床肺科杂志
臨床肺科雜誌
림상폐과잡지
JOUNAL OF CLINICAL PULMONARY MEDICINE
2015年
3期
423-425
,共3页
杨秉芬%王心静%蒋静%翟斐%曹志红%程小星
楊秉芬%王心靜%蔣靜%翟斐%曹誌紅%程小星
양병분%왕심정%장정%적비%조지홍%정소성
环介导等温扩增法%荧光实时定量PCR%结核病
環介導等溫擴增法%熒光實時定量PCR%結覈病
배개도등온확증법%형광실시정량PCR%결핵병
loop-mediated isothermal amplification%real-time PCR%tuberculosis
目的:研究环介导等温扩增法在不同临床样本中的结核病诊断价值。方法使用环介导等温扩增法检测肺泡灌洗液、局部穿刺液、腹水和脑脊液中的结核分枝杆菌复合群的特异性基因IS1081,并与实时荧光定量PCR进行比较。结果在荷菌量较高的肺泡灌洗液和局部穿刺液中,环介导等温扩增法敏感性比实时荧光定量PCR低(80% vs 92%);在荷菌量极低的腹水和脑脊液中,环介导等温扩增法敏感性比实时荧光定量PCR高(34.6% vs 15.4%),但特异性略有下降(80% vs 93.3%);综合计算所有样本的敏感性,环介导等温扩增法与实时荧光定量PCR无显著差别(56.9% vs 52.9%)。结论环介导等温扩增法具有较高敏感性和特异性,可以用于结核病诊断。
目的:研究環介導等溫擴增法在不同臨床樣本中的結覈病診斷價值。方法使用環介導等溫擴增法檢測肺泡灌洗液、跼部穿刺液、腹水和腦脊液中的結覈分枝桿菌複閤群的特異性基因IS1081,併與實時熒光定量PCR進行比較。結果在荷菌量較高的肺泡灌洗液和跼部穿刺液中,環介導等溫擴增法敏感性比實時熒光定量PCR低(80% vs 92%);在荷菌量極低的腹水和腦脊液中,環介導等溫擴增法敏感性比實時熒光定量PCR高(34.6% vs 15.4%),但特異性略有下降(80% vs 93.3%);綜閤計算所有樣本的敏感性,環介導等溫擴增法與實時熒光定量PCR無顯著差彆(56.9% vs 52.9%)。結論環介導等溫擴增法具有較高敏感性和特異性,可以用于結覈病診斷。
목적:연구배개도등온확증법재불동림상양본중적결핵병진단개치。방법사용배개도등온확증법검측폐포관세액、국부천자액、복수화뇌척액중적결핵분지간균복합군적특이성기인IS1081,병여실시형광정량PCR진행비교。결과재하균량교고적폐포관세액화국부천자액중,배개도등온확증법민감성비실시형광정량PCR저(80% vs 92%);재하균량겁저적복수화뇌척액중,배개도등온확증법민감성비실시형광정량PCR고(34.6% vs 15.4%),단특이성략유하강(80% vs 93.3%);종합계산소유양본적민감성,배개도등온확증법여실시형광정량PCR무현저차별(56.9% vs 52.9%)。결론배개도등온확증법구유교고민감성화특이성,가이용우결핵병진단。
Obejective To study the diagnostic value of loop-mediated isothermal amplification( LAMP) in different tuberculosis clinical samples. Methods LAMP was used to detect mycobacterium tuberculosis complex-specific gene IS1081 in bronchoalveolar lavage fluid ( BALF ) , puncture fluid, ascites, and cerebrospinal fluid ( CSF) . And the result was compared with real-time PCR ( RT-PCR) . Results The sensitivity of LAMP was lower than RT-PCR (80% vs 92%) in BALF and puncture fluid, in which there were more bacteria. The sensitivity of LAMP was higher than RT-PCR (34. 6% vs 15. 4%) in ascites and CSF, in which the mount of bacteria was ex-tremely low, but the specificity of LAMP decreased slightly (80% vs 93. 3%). The sensitivities of LAMP and RT-PCR calculated in all samples showed no significant difference ( 56. 9% vs 52. 9%) . Conclusion LAMP has high sensitivity and specificity in detecting mycobacterium tuberculosis complex.