中国实用神经疾病杂志
中國實用神經疾病雜誌
중국실용신경질병잡지
CHINESE JOURNAL OF PRACTICAL NERVOUS DISEASES
2015年
2期
41-43
,共3页
姜黄素%miR-106a%anti-miR-106a%阿尔茨海默病
薑黃素%miR-106a%anti-miR-106a%阿爾茨海默病
강황소%miR-106a%anti-miR-106a%아이자해묵병
Curcumin%miR-106a%Anti-miR-106a%Alzheimer’s disease
目的：探讨阿尔茨海默病（Alzheimer’s disease ，AD）中姜黄素与miR‐106a的关系。方法将PC12细胞随机分为5组：正常对照组（PC12细胞组），模型组（PC12细胞与浓度为4μg／mL的Aβ1‐42共培养24 h），anti‐miR‐106a组（造模成功后转染anti‐miR‐106a），姜黄素组（造模成功后加姜黄素，终浓度为20μmol／L ）、共同作用组（anti‐miR‐106a加入姜黄素）。RT‐PCR检测miR‐106a的含量；Western blotting检测APP的含量。结果 RT‐PCR结果显示，与正常对照组相比，模型组和anti‐miR‐106a组miR‐106a含量均明显降低（P＜0.05），而姜黄素组明显升高（P＜0.05）。Western blotting结果显示，与正常对照组相比，模型组（P＜0.05）和anti‐miR‐106a组（P＜0.01）APP明显增加，姜黄素组 APP比模型组明显降低（P＜0.05）。结论姜黄素可通过正性调节miR‐106a而参与AD的治疗。
目的：探討阿爾茨海默病（Alzheimer’s disease ，AD）中薑黃素與miR‐106a的關繫。方法將PC12細胞隨機分為5組：正常對照組（PC12細胞組），模型組（PC12細胞與濃度為4μg／mL的Aβ1‐42共培養24 h），anti‐miR‐106a組（造模成功後轉染anti‐miR‐106a），薑黃素組（造模成功後加薑黃素，終濃度為20μmol／L ）、共同作用組（anti‐miR‐106a加入薑黃素）。RT‐PCR檢測miR‐106a的含量；Western blotting檢測APP的含量。結果 RT‐PCR結果顯示，與正常對照組相比，模型組和anti‐miR‐106a組miR‐106a含量均明顯降低（P＜0.05），而薑黃素組明顯升高（P＜0.05）。Western blotting結果顯示，與正常對照組相比，模型組（P＜0.05）和anti‐miR‐106a組（P＜0.01）APP明顯增加，薑黃素組 APP比模型組明顯降低（P＜0.05）。結論薑黃素可通過正性調節miR‐106a而參與AD的治療。
목적：탐토아이자해묵병（Alzheimer’s disease ，AD）중강황소여miR‐106a적관계。방법장PC12세포수궤분위5조：정상대조조（PC12세포조），모형조（PC12세포여농도위4μg／mL적Aβ1‐42공배양24 h），anti‐miR‐106a조（조모성공후전염anti‐miR‐106a），강황소조（조모성공후가강황소，종농도위20μmol／L ）、공동작용조（anti‐miR‐106a가입강황소）。RT‐PCR검측miR‐106a적함량；Western blotting검측APP적함량。결과 RT‐PCR결과현시，여정상대조조상비，모형조화anti‐miR‐106a조miR‐106a함량균명현강저（P＜0.05），이강황소조명현승고（P＜0.05）。Western blotting결과현시，여정상대조조상비，모형조（P＜0.05）화anti‐miR‐106a조（P＜0.01）APP명현증가，강황소조 APP비모형조명현강저（P＜0.05）。결론강황소가통과정성조절miR‐106a이삼여AD적치료。
Objective To investigate the relationship between curcumin and miR‐106a in Alzheimer’s disease.Methods PC12 cells were randomly divided into 5 groups, namely, normal control group, model group (cultured with 4 μg/mL Abeta1‐42 for 24 hours), anti‐miR‐106a group ( transfected with anti‐miR‐106a), curcumin group (adding 20 μmol/L curcumin) and coaction group (adding curcumin and anti‐miR‐106a).The miR‐106a content of each group was detected by RT‐PCR and APP content was detected by Western blotting.Results RT‐PCR showed that the concentration of miR‐106a decreased significantly in model group and anti‐miR‐106a group but increased markedly in curcumin group compared with normal control group.Western‐blotting showed that the APP content increased significantly in APP model group and anti‐miR‐106a group but decreased markedly in curcumin group compared with normal control group.Conclusion Curcumin maybe positively regulate miR‐106a in alzheimer’s disease.
