南方医科大学学报
南方醫科大學學報
남방의과대학학보
JOURNAL OF SOUTHERN MEDICAL UNIVERSITY
2015年
2期
284-287,291
,共5页
扶招弟%周丽芬%黄建荣%郭淑仪%章洁纯%方永标%刘筱蔼%陈庆梓%李建华
扶招弟%週麗芬%黃建榮%郭淑儀%章潔純%方永標%劉篠藹%陳慶梓%李建華
부초제%주려분%황건영%곽숙의%장길순%방영표%류소애%진경재%리건화
瞬时感受器电位通道1%臭氧%肺%炎症
瞬時感受器電位通道1%臭氧%肺%炎癥
순시감수기전위통도1%취양%폐%염증
transient receptor potential canonical 1%ozone%lung%inflammation
目的:探讨经典瞬时感受器电位通道1(TRPC1)在臭氧暴露下小鼠肺组织中的表达情况。方法采用臭氧暴露建立小鼠肺组织炎症模型,通过对小鼠行为学观察、支气管肺泡灌洗液的细胞分类计数和肺组织病理形态分析证实模型建立成功后,RT-PCR法检测肺组织TRPC1 mRNA的表达,Western blot法和免疫组织化学染色法分别观察TRPC1蛋白在肺组织的表达和定位情况。结果 RT-PCR和Western blot结果显示,与对照组相比,臭氧组小鼠肺组织TRPC1 mRNA及其蛋白表达上调(P<0.05);免疫组织化学染色法进一步证实,臭氧组小鼠肺组织TRPC1蛋白在肺泡上皮细胞、支气管上皮细胞、浸润的炎症细胞的表达均较对照组明显增强(P<0.05);相关性分析显示,小鼠肺组织中TRPC1 mRNA和蛋白的表达均与支气管肺泡灌洗液中白细胞总数、巨噬细胞、中性粒细胞、淋巴细胞数呈显著性正相关(P<0.01)。结论 TRPC1可能与小鼠肺组织炎症发病机制有关。
目的:探討經典瞬時感受器電位通道1(TRPC1)在臭氧暴露下小鼠肺組織中的錶達情況。方法採用臭氧暴露建立小鼠肺組織炎癥模型,通過對小鼠行為學觀察、支氣管肺泡灌洗液的細胞分類計數和肺組織病理形態分析證實模型建立成功後,RT-PCR法檢測肺組織TRPC1 mRNA的錶達,Western blot法和免疫組織化學染色法分彆觀察TRPC1蛋白在肺組織的錶達和定位情況。結果 RT-PCR和Western blot結果顯示,與對照組相比,臭氧組小鼠肺組織TRPC1 mRNA及其蛋白錶達上調(P<0.05);免疫組織化學染色法進一步證實,臭氧組小鼠肺組織TRPC1蛋白在肺泡上皮細胞、支氣管上皮細胞、浸潤的炎癥細胞的錶達均較對照組明顯增彊(P<0.05);相關性分析顯示,小鼠肺組織中TRPC1 mRNA和蛋白的錶達均與支氣管肺泡灌洗液中白細胞總數、巨噬細胞、中性粒細胞、淋巴細胞數呈顯著性正相關(P<0.01)。結論 TRPC1可能與小鼠肺組織炎癥髮病機製有關。
목적:탐토경전순시감수기전위통도1(TRPC1)재취양폭로하소서폐조직중적표체정황。방법채용취양폭로건립소서폐조직염증모형,통과대소서행위학관찰、지기관폐포관세액적세포분류계수화폐조직병리형태분석증실모형건립성공후,RT-PCR법검측폐조직TRPC1 mRNA적표체,Western blot법화면역조직화학염색법분별관찰TRPC1단백재폐조직적표체화정위정황。결과 RT-PCR화Western blot결과현시,여대조조상비,취양조소서폐조직TRPC1 mRNA급기단백표체상조(P<0.05);면역조직화학염색법진일보증실,취양조소서폐조직TRPC1단백재폐포상피세포、지기관상피세포、침윤적염증세포적표체균교대조조명현증강(P<0.05);상관성분석현시,소서폐조직중TRPC1 mRNA화단백적표체균여지기관폐포관세액중백세포총수、거서세포、중성립세포、림파세포수정현저성정상관(P<0.01)。결론 TRPC1가능여소서폐조직염증발병궤제유관。
Objective To detect the expression of transient receptor potential canonical 1 (TRPC1) in a mouse model of ozone-induced lung inflammation and explore its role in lung inflammation. Methods In a mouse model of lung inflammation established by ozone exposure, the expression of TRPC1 in the inflammatory lung tissues was detected by RT-PCR, Wstern blotting and immunohistochemistry. Results Compared to the control mice, the mice exposed to ozone showed significantly increased expression level of TRPC1 mRNA and protein in the inflammatory lung tissues (P<0.05). Immunohistochemistry showed increased TRPC1 protein expressions in the alveolar epithelial cells, bronchial epithelial cells, and inflammatory cells in the inflammatory lung tissues (P<0.05). The mRNA and protein expression levels of TRPC1 were positively correlated with the counts of white blood cells, macrophages, neutrophils and lymphocytes in the bronchoalveolar lavage fluid of the exposed mice (P<0.01). Conclusion TRPC1 may play a role in ozone-induced lung inflammation in mice.