南方医科大学学报
南方醫科大學學報
남방의과대학학보
JOURNAL OF SOUTHERN MEDICAL UNIVERSITY
2015年
2期
281-283
,共3页
生存素%短发夹状RNA%子宫内膜异位症%慢病毒%裸鼠
生存素%短髮夾狀RNA%子宮內膜異位癥%慢病毒%裸鼠
생존소%단발협상RNA%자궁내막이위증%만병독%라서
survivin%short hairpin RNA%endometriosis%lentivirus%nude mice
目的：研究慢病毒介导生存素(survivin)基因特异性短发夹状RNA(shRNA)对人子宫内膜在裸鼠腹腔种植生长的影响。方法将45只裸鼠随机分为实验组、阴性对照组及空白对照组，每组15只，将子宫内膜异位症患者在位内膜注射入裸鼠腹腔，同时将survivin-shRNA慢病毒、空载慢病毒及磷酸盐缓冲液分别注射至3组裸鼠腹腔内，2周后观察各组子宫内膜种植成功率，光镜下观察异位病灶的形态学特点，免疫组化方法检测各组病灶中survivin的表达水平。结果实验组子宫内膜种植成功率（26.67%）明显低于阴性对照组（73.33%）及空白对照组（80%，P<0.01）；实验组成活的异位内膜腺体发育不良，间质伴有不同程度的坏死；该组病灶中survivin蛋白表达亦明显下降，与两对照组比较差异有显著性（P<0.001）。结论慢病毒介导survivin基因特异性shRNA能够通过靶向沉默survivin基因明显抑制人子宫内膜在裸鼠腹腔中的种植生长。
目的：研究慢病毒介導生存素(survivin)基因特異性短髮夾狀RNA(shRNA)對人子宮內膜在裸鼠腹腔種植生長的影響。方法將45隻裸鼠隨機分為實驗組、陰性對照組及空白對照組，每組15隻，將子宮內膜異位癥患者在位內膜註射入裸鼠腹腔，同時將survivin-shRNA慢病毒、空載慢病毒及燐痠鹽緩遲液分彆註射至3組裸鼠腹腔內，2週後觀察各組子宮內膜種植成功率，光鏡下觀察異位病竈的形態學特點，免疫組化方法檢測各組病竈中survivin的錶達水平。結果實驗組子宮內膜種植成功率（26.67%）明顯低于陰性對照組（73.33%）及空白對照組（80%，P<0.01）；實驗組成活的異位內膜腺體髮育不良，間質伴有不同程度的壞死；該組病竈中survivin蛋白錶達亦明顯下降，與兩對照組比較差異有顯著性（P<0.001）。結論慢病毒介導survivin基因特異性shRNA能夠通過靶嚮沉默survivin基因明顯抑製人子宮內膜在裸鼠腹腔中的種植生長。
목적：연구만병독개도생존소(survivin)기인특이성단발협상RNA(shRNA)대인자궁내막재라서복강충식생장적영향。방법장45지라서수궤분위실험조、음성대조조급공백대조조，매조15지，장자궁내막이위증환자재위내막주사입라서복강，동시장survivin-shRNA만병독、공재만병독급린산염완충액분별주사지3조라서복강내，2주후관찰각조자궁내막충식성공솔，광경하관찰이위병조적형태학특점，면역조화방법검측각조병조중survivin적표체수평。결과실험조자궁내막충식성공솔（26.67%）명현저우음성대조조（73.33%）급공백대조조（80%，P<0.01）；실험조성활적이위내막선체발육불량，간질반유불동정도적배사；해조병조중survivin단백표체역명현하강，여량대조조비교차이유현저성（P<0.001）。결론만병독개도survivin기인특이성shRNA능구통과파향침묵survivin기인명현억제인자궁내막재라서복강중적충식생장。
Objective To investigate the inhibitory effect of lentiviral vector-mediated short hairpin RNA targeting survivin (LV-survivin shRNA) on the growth of human endometrium xenograft in the abdominal cavity of nude mice. Methods The endometrium xenografts from 8 women with endometriosis were injected into the peritoneal cavities of 45 nude mice. The mice were then randomly assigned to receive intraperitoneal injection of LV-survivin shRNA, pGCL-NC-GFP (negative control) or PBS (blank control). Two weeks later, the number and morphometry of endometriotic lesions were quantified and the expression of survivin protein were detected by immunohistochemistry. Results The formation of endometriotic lesions was significantly suppressed in mice receiving LV-survivin shRNA injection as compared with those in the two control groups (P<0.001). The mice in LV-survivin-shRNA group showed significantly down-regulated expression levels of survivin protein compared with those in the negative and blank control groups, presenting also necrosis in the endometriosis-like lesions in microscopic observation. Conclusion Lentiviral vector-mediated shRNA can effectively inhibit the expression of survivin in human endometrium xengrafts and suppress the formation and growth of endometriotic lesions in the abdominal cavities of nude mice.
