CAJ | 학술논문

目的：研究硼酸-硼砂缓冲液制备的尿酸酶-过氧化氢酶脂质体（BUCLP）中尿酸酶的体外特性。方法采用逆向蒸发法制备BUCLP，并考察其部分理化性质特性。结果 BUCLP中尿酸酶最适温度保持在40℃，最适pH值由8.5降为8.0，而Km值也由14.207μmol/L降为13.623μmol/L；尿酸酶-过氧化氢酶（UAC）与空白纳米脂质体作用后，再与FITC结合，其荧光强度高于游离UAC与FITC结合的荧光强度，且BUCLP在280 nm处的荧光强度高于游离UAC。结论尿酸酶和过氧化氢酶联合制备成BUCLP后尿酸酶的活性增加。
목적：연구붕산-붕사완충액제비적뇨산매-과양화경매지질체（BUCLP）중뇨산매적체외특성。방법채용역향증발법제비BUCLP，병고찰기부분이화성질특성。결과 BUCLP중뇨산매최괄온도보지재40℃，최괄pH치유8.5강위8.0，이Km치야유14.207μmol/L강위13.623μmol/L；뇨산매-과양화경매（UAC）여공백납미지질체작용후，재여FITC결합，기형광강도고우유리UAC여FITC결합적형광강도，차BUCLP재280 nm처적형광강도고우유리UAC。결론뇨산매화과양화경매연합제비성BUCLP후뇨산매적활성증가。
Objective To characterize the property of uricase loaded in uricase-catalase liposomes (BUCLPs) prepared using borate buffer. Methods BUCLPs were prepared using reverse-phase evaporation, and the physicochemical properties of uricase in the prepared BUCLPs were examined. Results The optimal temperature of BUCLP and URI was 40 oC, their optimal pH values were 8.0 and 8.5, and their Michaelis-Menten constants were 14.207 μmol/L and 13.623 μmol/L, respectively. Fluorescence intensity of nanoliposome-loaded uricase-catalase that bound to FITC was higher than that of uricase-catalase binding directly with FITC; the fluorescence intensity of BUCLP was higher than that of free uricase-catalase at 280 nm. Conclusion Uricase activity is enhanced after loading in uricase and catalase liposomes.