临床荟萃
臨床薈萃
림상회췌
CLINICAL FOCUS
2015年
2期
206-209
,共4页
胃癌%胞间黏附分子 1%免疫组织化学%蛋白质印迹
胃癌%胞間黏附分子 1%免疫組織化學%蛋白質印跡
위암%포간점부분자 1%면역조직화학%단백질인적
gastric carcinoma%intercellular adhesion molecule-1%immunohistochemistry%Western blot
目的：研究细胞间黏附分子1(ICAM-1)在胃癌组织、癌旁胃组织及胃癌细胞系中的表达及对临床实践的价值。方法从90例胃癌组织、40例癌旁胃组织及2种胃癌细胞系(MKN45、SGC-7901)中提取蛋白质,以蛋白质印迹法检测 ICAM-1的表达水平；对90例胃癌组织以 ICAM-1为一抗进行免疫组织化学(IHC)检测,并根据 IHC 结果把患者分为 ICAM-1阳性组(68例)和 ICAM-1阴性组(22例)。结果胃癌组织及胃癌细胞系中 ICAM-1的表达水平(4.5±0.3)、(5.6±0.2)高于癌旁胃组织(1.0±0.1),差异具有统计学意义(P <0.01)。胃癌组织及癌旁胃组织中 ICAM-1阳性率分别为75.6%(68/90)、32.5%(13/40),差异具有统计学意义(P <0.01)；与 ICAM-1阴性组比较, ICAM-1阳性组肿瘤大小、肿瘤浸润深度、神经周围浸润率均增高,差异均具有统计学意义(P <0.01)；ICAM-1阳性组复发率高于 ICAM 阴性组(67.6% vs 22.7%),5年存活率则低于 ICAM-1阴性组(61.1% vs 80.0%),差异均具有统计学意义(P <0.05)。结论 ICAM-1在胃癌中的过表达提示癌肿侵袭性增强,预后差。
目的：研究細胞間黏附分子1(ICAM-1)在胃癌組織、癌徬胃組織及胃癌細胞繫中的錶達及對臨床實踐的價值。方法從90例胃癌組織、40例癌徬胃組織及2種胃癌細胞繫(MKN45、SGC-7901)中提取蛋白質,以蛋白質印跡法檢測 ICAM-1的錶達水平；對90例胃癌組織以 ICAM-1為一抗進行免疫組織化學(IHC)檢測,併根據 IHC 結果把患者分為 ICAM-1暘性組(68例)和 ICAM-1陰性組(22例)。結果胃癌組織及胃癌細胞繫中 ICAM-1的錶達水平(4.5±0.3)、(5.6±0.2)高于癌徬胃組織(1.0±0.1),差異具有統計學意義(P <0.01)。胃癌組織及癌徬胃組織中 ICAM-1暘性率分彆為75.6%(68/90)、32.5%(13/40),差異具有統計學意義(P <0.01)；與 ICAM-1陰性組比較, ICAM-1暘性組腫瘤大小、腫瘤浸潤深度、神經週圍浸潤率均增高,差異均具有統計學意義(P <0.01)；ICAM-1暘性組複髮率高于 ICAM 陰性組(67.6% vs 22.7%),5年存活率則低于 ICAM-1陰性組(61.1% vs 80.0%),差異均具有統計學意義(P <0.05)。結論 ICAM-1在胃癌中的過錶達提示癌腫侵襲性增彊,預後差。
목적：연구세포간점부분자1(ICAM-1)재위암조직、암방위조직급위암세포계중적표체급대림상실천적개치。방법종90례위암조직、40례암방위조직급2충위암세포계(MKN45、SGC-7901)중제취단백질,이단백질인적법검측 ICAM-1적표체수평；대90례위암조직이 ICAM-1위일항진행면역조직화학(IHC)검측,병근거 IHC 결과파환자분위 ICAM-1양성조(68례)화 ICAM-1음성조(22례)。결과위암조직급위암세포계중 ICAM-1적표체수평(4.5±0.3)、(5.6±0.2)고우암방위조직(1.0±0.1),차이구유통계학의의(P <0.01)。위암조직급암방위조직중 ICAM-1양성솔분별위75.6%(68/90)、32.5%(13/40),차이구유통계학의의(P <0.01)；여 ICAM-1음성조비교, ICAM-1양성조종류대소、종류침윤심도、신경주위침윤솔균증고,차이균구유통계학의의(P <0.01)；ICAM-1양성조복발솔고우 ICAM 음성조(67.6% vs 22.7%),5년존활솔칙저우 ICAM-1음성조(61.1% vs 80.0%),차이균구유통계학의의(P <0.05)。결론 ICAM-1재위암중적과표체제시암종침습성증강,예후차。
Objective To observe the expression of intercellular adhesion molecule-1 (ICAM-1 )in gastric carcinoma and its adjacent tissues and two cultured gastric carcinoma cell lines,so as to explore its value in the clinical practice.Methods The expression of ICAM-1 in 90 cases of gastric carcinoma tissues,40 cases of adjacent tissues and two cultured gastric carcinoma cell lines (MKN45 and SGC-7901 ) was determined by Western blot and immunohistochemistry(IHC).The gastric carcinoma tissues were then divided into ICAM-1-positive group (n =68) and ICAM-1-negative group (n =22).Results The expression of ICAM-1 in gastric carcinoma tissues (4.5±0.3)and gastric carcinoma cell lines (5.6 ± 0.2 )were significantly higher than that in the adjacent tissues (1.0 ± 0.1,P <0.01).The positive rate of ICAM-1 in gastric carcinoma tissues was 75.6%(68/90),remarkably higher than that in the adjacent tissues 32.5%(13/40)(P <0.01).Compared with those in the ICAM-1-negative group,ICAM-1-positive group had larger tumor size,more serious infiltration and more common nerve invasion (P <0.01).Furthermore,the recurrence rate in the ICAM-1-positive group was markedly higher than that in the ICAM-1-negative group (67.6% vs 22.7%,P <0.05)and the five year survival rate in the ICAM-1-positive group was prominently lower than that in the ICAM-1-negative group (61.1% vs 80.0%,P <0.05).Conclusion The overexpression of ICAM-1 in gastric cancer tissues predicted more intensive invasion and poorer prognosis.
