CAJ | 학술논문

目的：建立甲型 H1N1流感疫苗神经氨酸酶含量测定参考品。方法对甲型 H1N1流感疫苗原液进行还原电泳后，采用免疫印迹，糖蛋白染色，方法初步确定甲流 H1N1疫苗原液中神经氨酸酶 SDS-PAGE 条带位置，切取条带后通过 edman N 端测序法进行确认。采用 Lowry 法进行总蛋白定量，SDS-PAGE 密度扫描的方法确定神经氨酸酶比例，计算出疫苗原液中神经氨酸酶含量。结果确定甲流疫苗原液中神经氨酸酶在 SDS-PAGE 中相对分子质量约71000，对多批次疫苗原液进行测定后，选取神经氨酸酶含量较高的批次进行测定，总蛋白质量浓度为1046．00μg／mL，神经氨酸酶质量分数11．78％。二者相乘得出该批次疫苗原液神经氨酸酶含量为123．22μg／mL。结论研究成功建立了甲型 H1N1流感疫苗神经氨酸酶含量测定参考品，其他毒株生产的流感疫苗也可以参考该方法建立 NA 含量测定参考品。
목적：건립갑형 H1N1류감역묘신경안산매함량측정삼고품。방법대갑형 H1N1류감역묘원액진행환원전영후，채용면역인적，당단백염색，방법초보학정갑류 H1N1역묘원액중신경안산매 SDS-PAGE 조대위치，절취조대후통과 edman N 단측서법진행학인。채용 Lowry 법진행총단백정량，SDS-PAGE 밀도소묘적방법학정신경안산매비례，계산출역묘원액중신경안산매함량。결과학정갑류역묘원액중신경안산매재 SDS-PAGE 중상대분자질량약71000，대다비차역묘원액진행측정후，선취신경안산매함량교고적비차진행측정，총단백질량농도위1046．00μg／mL，신경안산매질량분수11．78％。이자상승득출해비차역묘원액신경안산매함량위123．22μg／mL。결론연구성공건립료갑형 H1N1류감역묘신경안산매함량측정삼고품，기타독주생산적류감역묘야가이삼고해방법건립 NA 함량측정삼고품。
Objective To establish a reference material for qualification of the neuraminidase content in the pandemic H1N1 influenza vaccine.Methods Proteins of pandemic influenza H1N1 virus were separated by reducing SDS-PAGE. To take out the staining band of neuraminidase in SDS-PAGE,it was identified by Western blot,Glycoprotein staining and edman degradation method.Lowry protein assay and SDS-PAGE in combination with densitometry analysis were used to quantify the neuraminidase contents in influenza H1N1 vaccine bulk.Results The neuraminidase band in SDS-PAGE was about MW 71 000.The neuraminidase proportion of multiple lots of H1N1 influenza vaccine bulk were tested.Vaccine bulk lots with some higher neuraminidase propotions were chosen for neuraminidase quantification,and the total proteins were de-terminated as 1 046.00 μg/mL and the neuraminidase content acounted for 123.22 μg/mL(11.78%).Conclusion The reference material for qualification of neuraminidase content in the H1N1 influenza vaccine was successfully established in this study,in a same way to develop the reference materials of NA content in preparation of other influenza strain vaccines.