疑难病杂志
疑難病雜誌
의난병잡지
JOURNAL OF DIFFICULT AND COMPLICATED CASES
2015年
2期
141-144
,共4页
徐伟乐%李辉%秦学博%侯宏伟%齐海亮
徐偉樂%李輝%秦學博%侯宏偉%齊海亮
서위악%리휘%진학박%후굉위%제해량
结核性脓胸%CCR2基因%CCR5基因%单核苷酸多态性
結覈性膿胸%CCR2基因%CCR5基因%單覈苷痠多態性
결핵성농흉%CCR2기인%CCR5기인%단핵감산다태성
Tuberculous empyema%CCR2 gene%CCR5 gene%Single nucleotide polymorphism
目的：探讨CCR2基因-190 G/A和CCR5基因-59029 G/A单核苷酸多态性( SNP)与结核性脓胸的关系。方法采用聚合酶链反应—限制性片段长度多态性( PCR-RFLP)方法对结核性脓胸患者300例和健康对照组300例进行CCR2基因-190 G/A和CCR5基因-59029 G/A SNPs检测。结果与CCR2基因-190 GG基因型相比,携带AG和AA基因型均可增加结核性脓胸发病风险( OR=2.254,95%CI 1.043~4.868；OR=8.728,95%CI 4.224~18.033)。进行分层分析发现,无卡介苗( BCG)接种史、体质量指数( BMI)<18.5 kg/m2均增加结核性脓胸发病风险(OR=3.309,95%CI 2.108~4.382；OR=2.767,95%CI 1.918~3.993)。 CCR5基因-59029 G/A 各基因型未入选回归方程,无统计学意义( P >0.05)。结论 CCR2基因-190 G/A SNP可能与结核性脓胸的发病风险有关；CCR5基因-59029 G/A SNP可能与结核性脓胸无关。
目的：探討CCR2基因-190 G/A和CCR5基因-59029 G/A單覈苷痠多態性( SNP)與結覈性膿胸的關繫。方法採用聚閤酶鏈反應—限製性片段長度多態性( PCR-RFLP)方法對結覈性膿胸患者300例和健康對照組300例進行CCR2基因-190 G/A和CCR5基因-59029 G/A SNPs檢測。結果與CCR2基因-190 GG基因型相比,攜帶AG和AA基因型均可增加結覈性膿胸髮病風險( OR=2.254,95%CI 1.043~4.868；OR=8.728,95%CI 4.224~18.033)。進行分層分析髮現,無卡介苗( BCG)接種史、體質量指數( BMI)<18.5 kg/m2均增加結覈性膿胸髮病風險(OR=3.309,95%CI 2.108~4.382；OR=2.767,95%CI 1.918~3.993)。 CCR5基因-59029 G/A 各基因型未入選迴歸方程,無統計學意義( P >0.05)。結論 CCR2基因-190 G/A SNP可能與結覈性膿胸的髮病風險有關；CCR5基因-59029 G/A SNP可能與結覈性膿胸無關。
목적：탐토CCR2기인-190 G/A화CCR5기인-59029 G/A단핵감산다태성( SNP)여결핵성농흉적관계。방법채용취합매련반응—한제성편단장도다태성( PCR-RFLP)방법대결핵성농흉환자300례화건강대조조300례진행CCR2기인-190 G/A화CCR5기인-59029 G/A SNPs검측。결과여CCR2기인-190 GG기인형상비,휴대AG화AA기인형균가증가결핵성농흉발병풍험( OR=2.254,95%CI 1.043~4.868；OR=8.728,95%CI 4.224~18.033)。진행분층분석발현,무잡개묘( BCG)접충사、체질량지수( BMI)<18.5 kg/m2균증가결핵성농흉발병풍험(OR=3.309,95%CI 2.108~4.382；OR=2.767,95%CI 1.918~3.993)。 CCR5기인-59029 G/A 각기인형미입선회귀방정,무통계학의의( P >0.05)。결론 CCR2기인-190 G/A SNP가능여결핵성농흉적발병풍험유관；CCR5기인-59029 G/A SNP가능여결핵성농흉무관。
Objective To investigate the relationship of CCR2 gene-190 G/A, CCR5 gene-59029 G/A single nucle-otide polymorphism ( SNP) and tuberculous empyema. Methods Using polymerase chain reaction restriction fragment length polymorphism ( PCR-RFLP) method, 300 cases of tuberculous empyema patients and 300 healthy control were received 190 G/A and 59029 G/A SNPs detection. Results Compared to CCR2 gene-190 GG genotype, carrying AG and AA genotype can increase the risk of tuberculous empyema ( OR =2. 254, 95%CI 1. 043 -4. 868; OR =8. 728, 95%CI 4. 224 -18. 033). Stratified analysis revealed that, without Bacillus Calmette Guerin (BCG) vaccination history, body mass index (BMI) <18. 5 kg/m2 were all increased the risk of tuberculous empyema (OR=3. 309, 95%CI 2. 108 -4. 382; OR=2. 767, 95%CI 1. 918-3. 993). CCR5 gene-59029 G/A genotypes were not selected in the regression equation, no statisti-cal significance was found ( P >0. 05). Conclusion CCR2 gene-190 G/A SNP might be related to the tuberculous empye-ma. CCR5 gene-59029 G/A SNP may have no association with tuberculous empyema.
