CAJ | 학술논문

目的：观察大黄素对博莱霉素所致大鼠肺纤维化的影响,并探讨其保护机制。方法将60只♂ SD大鼠随机分为正常对照组、假手术组、模型组、大黄素低剂量干预组、大黄素高剂量干预组和泼尼松组,每组10只,后4组经气管内注入博莱霉素建立大鼠肺纤维化模型,从d2开始,低、高剂量干预组大鼠分别予20、80 mg · kg-1大黄素2 mL灌胃,泼尼松组以5 mg·kg-1醋酸泼尼松2 mL灌胃,其余2组予2 mL生理盐水灌胃,d 28处死所有大鼠,取出肺组织行HE染色和Masson 染色,测定肺组织羟脯氨酸( HYP )、丙二醛( MDA)、超氧化物歧化酶( SOD )、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)含量,采用酶联免疫吸附法(ELISA)测定血清肿瘤坏死因子-α(TNF-α)、白介素(IL)-6、IL-17浓度,通过Western blot分析肺组织中Kelch样ECH联合蛋白1( Keap 1)、NF-E2相关因子2( Nrf2)、核因子-κB ( NF-κB) p65表达。结果低、高剂量干预组及泼尼松组肺泡炎症和肺纤维化程度明显轻于模型组( P <0．05或 P <0．01)。与正常对照组或假手术组比较,模型组肺组织HYP、MDA 含量、胞核 Nrf2、NF-κB p65表达水平及血清TNF-α、IL-6、IL-17浓度增加( P <0．01),而肺组织 SOD、GSH-Px、CAT含量及胞质Keap 1表达水平降低( P<0．01)。经低、高剂量大黄素或泼尼松处理后,肺组织HYP、MDA含量、胞质Keap 1表达水平、胞核NF-κB p65表达水平及血清TNF-α、IL-6、IL-17浓度减少,肺组织SOD、GSH-Px、CAT含量及胞核Nrf2表达水平升高,与模型组比较,差异均有显著性(P<0．01)。高剂量干预组、泼尼松组上述指标改善程度优于低剂量干预组(P<0．05),但高剂量干预组与泼尼松组比较,差异无显著性(P>0．05)。结论大黄素可能通过增强抗氧化能力及抑制炎症反应对肺纤维化大鼠产生保护作用。
목적：관찰대황소대박래매소소치대서폐섬유화적영향,병탐토기보호궤제。방법장60지♂ SD대서수궤분위정상대조조、가수술조、모형조、대황소저제량간예조、대황소고제량간예조화발니송조,매조10지,후4조경기관내주입박래매소건립대서폐섬유화모형,종d2개시,저、고제량간예조대서분별여20、80 mg · kg-1대황소2 mL관위,발니송조이5 mg·kg-1작산발니송2 mL관위,기여2조여2 mL생리염수관위,d 28처사소유대서,취출폐조직행HE염색화Masson 염색,측정폐조직간포안산( HYP )、병이철( MDA)、초양화물기화매( SOD )、곡광감태과양화물매(GSH-Px)、과양화경매(CAT)함량,채용매련면역흡부법(ELISA)측정혈청종류배사인자-α(TNF-α)、백개소(IL)-6、IL-17농도,통과Western blot분석폐조직중Kelch양ECH연합단백1( Keap 1)、NF-E2상관인자2( Nrf2)、핵인자-κB ( NF-κB) p65표체。결과저、고제량간예조급발니송조폐포염증화폐섬유화정도명현경우모형조( P <0．05혹 P <0．01)。여정상대조조혹가수술조비교,모형조폐조직HYP、MDA 함량、포핵 Nrf2、NF-κB p65표체수평급혈청TNF-α、IL-6、IL-17농도증가( P <0．01),이폐조직 SOD、GSH-Px、CAT함량급포질Keap 1표체수평강저( P<0．01)。경저、고제량대황소혹발니송처리후,폐조직HYP、MDA함량、포질Keap 1표체수평、포핵NF-κB p65표체수평급혈청TNF-α、IL-6、IL-17농도감소,폐조직SOD、GSH-Px、CAT함량급포핵Nrf2표체수평승고,여모형조비교,차이균유현저성(P<0．01)。고제량간예조、발니송조상술지표개선정도우우저제량간예조(P<0．05),단고제량간예조여발니송조비교,차이무현저성(P>0．05)。결론대황소가능통과증강항양화능력급억제염증반응대폐섬유화대서산생보호작용。
Aim To observe the influence of emodin on bleomycin-induced pulmonary fibrosis in rats, and explore its protective mechanisms. Methods Sixty SD rats were randomly divided into normal control group, sham operation group, model group, low-dose inter-vention group, high-dose intervention group and pred-nisone group. Each group included 10 animals. Rats in the latter 4 groups were intratracheally administered with bleomycin to establish pulmonary fibrosis model. From the second day, rats in low-and high-dose inter-vention groups were intragastrically treated with 2 mL of 20 and 80 mg · kg-1 emodin, respectively. Predni-sone group were intragastrically administrated with 2 mL of 5 mg·kg-1 prednisone acetate. However, con-trol and model groups were treated with 2 mL of normal saline. All rats were sacrificed on day 28. Pulmonary tissues were then removed, and HE and Masson stai-ning were performed. The contents of hydroxyproline ( HYP ) , malondialdehyde ( MDA ) , superoxide dis-mutase ( SOD) , glutathione-peroxidase ( GSH-Px) and catalase (CAT) in pulmonary tissues were measured. Serum concentrations of tumor necrosis factor-α ( TNF-α) , interleukin ( IL )-6 and IL-17 were detected by enzyme linked immunosorbent assay ( ELISA ) . The expression of Kelch-like ECH-associated protein 1 ( Keap 1 ) , nuclear factor-erythroid 2-related factor 2 (Nrf2) and nuclear factor-κB (NF-κB) p65 in pulmo-nary tissues was analyzed using Western blot. Results The pulmonary inflammation and fibrosis in low-and high-dose intervention as well as prednisone groups was significantly improved when compared with model group ( P<0. 05 , 0. 01 ) . In comparison with normal control group or sham operation group, pulmonary HYP and MDA contents, Nrf2 and NF-κB p65 expression levels in the nucleus, and serum concentrations of TNF-α, IL-6 and IL-17 were increased ( P <0. 01 ) , but pulmonary SOD, GSH-Px and CAT contents and Keap 1 expression levels in the cytoplasm were de-creased ( P <0. 01 ) in model group. Upon treatment with low-and high-dose emodin or prednisone, pulmo-nary HYP and MDA contents, Keap 1 expression levels in the cytoplasm, NF-κB p65 expression levels in the nucleus , and serum concentrations of TNF-α, IL-6 and IL-17 were reduced while pulmonary SOD, GSH-Px and CAT contents and Nrf2 expression levels in the nu-cleus were enhanced as compared to model group ( P<0. 01 ) . The above indicators were significantly im-proved in high-dose intervention and prednisone groups compared with low-dose intervention group ( P <0. 05). Nevertheless, There was no significant differ-ence between high-dose intervention group and predni-sone group ( P >0. 05 ) . Conclusion Emodin may protect against rats with pulmonary fibrosis by enhan-cing antioxidative ability and inhibiting inflammatory response.