中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2015年
2期
237-240,241
,共5页
双团棘胸蛙%胸腺肽-β4%皮肤%cDNA 序列%两栖动物%鸡胚绒毛尿囊膜%人血管内皮细胞
雙糰棘胸蛙%胸腺肽-β4%皮膚%cDNA 序列%兩棲動物%鷄胚絨毛尿囊膜%人血管內皮細胞
쌍단극흉와%흉선태-β4%피부%cDNA 서렬%량서동물%계배융모뇨낭막%인혈관내피세포
Nanorana yunnanensis%thymosin-β4%skin%cDNA sequences%amphibians%chicken chorioal-lantoic membrane%human umbilical vein endothelial cells
目的：研究双团棘胸蛙( Nanorana yunnanensis)皮肤胸腺肽-β4的基因和促进血管生成作用。方法通过基因扩增的方法克隆出双团棘胸蛙的2个皮肤胸腺肽-β4( pTβ4-1和pTβ4-2)的cDNA；根据推导蛋白序列合成多肽在鸡胚尿囊膜和内皮细胞实验系统进行血管生成活性分析。结果 pTβ4-1和pTβ4-2的cDNA长度分别为662 bp和673 bp,开放阅读框都为135 bp,编码44个氨基酸残基。成熟蛋白拥有四足动物胸腺肽β4的典型特征。 pTβ4-1和pTβ4-2的cDNA序列及成熟蛋白与来源于人类、家鼠、家鸡、爪蟾、斑马鱼、大比目鱼和棕点湍蛙的胸腺肽-β4高度相似；合成的 pTβ4-1和pTβ4-2都能促进鸡胚尿囊膜血管生长和内皮细胞小管形成。结论双团棘胸蛙皮肤含有两个具有促血管生成的胸腺肽-β4。
目的：研究雙糰棘胸蛙( Nanorana yunnanensis)皮膚胸腺肽-β4的基因和促進血管生成作用。方法通過基因擴增的方法剋隆齣雙糰棘胸蛙的2箇皮膚胸腺肽-β4( pTβ4-1和pTβ4-2)的cDNA；根據推導蛋白序列閤成多肽在鷄胚尿囊膜和內皮細胞實驗繫統進行血管生成活性分析。結果 pTβ4-1和pTβ4-2的cDNA長度分彆為662 bp和673 bp,開放閱讀框都為135 bp,編碼44箇氨基痠殘基。成熟蛋白擁有四足動物胸腺肽β4的典型特徵。 pTβ4-1和pTβ4-2的cDNA序列及成熟蛋白與來源于人類、傢鼠、傢鷄、爪蟾、斑馬魚、大比目魚和棕點湍蛙的胸腺肽-β4高度相似；閤成的 pTβ4-1和pTβ4-2都能促進鷄胚尿囊膜血管生長和內皮細胞小管形成。結論雙糰棘胸蛙皮膚含有兩箇具有促血管生成的胸腺肽-β4。
목적：연구쌍단극흉와( Nanorana yunnanensis)피부흉선태-β4적기인화촉진혈관생성작용。방법통과기인확증적방법극륭출쌍단극흉와적2개피부흉선태-β4( pTβ4-1화pTβ4-2)적cDNA；근거추도단백서렬합성다태재계배뇨낭막화내피세포실험계통진행혈관생성활성분석。결과 pTβ4-1화pTβ4-2적cDNA장도분별위662 bp화673 bp,개방열독광도위135 bp,편마44개안기산잔기。성숙단백옹유사족동물흉선태β4적전형특정。 pTβ4-1화pTβ4-2적cDNA서렬급성숙단백여래원우인류、가서、가계、조섬、반마어、대비목어화종점단와적흉선태-β4고도상사；합성적 pTβ4-1화pTβ4-2도능촉진계배뇨낭막혈관생장화내피세포소관형성。결론쌍단극흉와피부함유량개구유촉혈관생성적흉선태-β4。
Aim To investigate the gene sequences and pro-angiogenic activities of thymosin-β4 from skin of Nanorana yunnanensis. Methods Two cDNA se-quences of thymosin-β4 from Nanorana yunnanensis designated as pTβ4-1 and pTβ4-2 were obtained by PCR. The tube formation and proliferation of human umbilical vein endothelial cells ( HUVEC ) and chick-en chorioallantoic membrane ( CAM) model were used to observe the pro-angiogenic effects of synthetic pep-tides according to the reduced amino acid sequences of pTβ4-1 and pTβ4-2 in vitro and in vivo, respectively. Results The cDNA sequences of Tβ4-1 and pTβ4-2 were composed of 662 bp and 673 bp, respectively, and their deduced amino acid sequences contained 44 residues which shared highly similarity with those from human , mouse , chicken , claw frog , zebra fish , turbot and Amolops loloensis. Furthermore, the mature amino acid sequences of pTβ4-1 and pTβ4-2 had highly con-served structural regions when compared with other thy-mosin-β4 previously reported. Synthetic peptides could significantly promote proliferation and tube formation of HUVEC and angiogenesis of CAM. Conclusion There are two thymosin-β4 peptides in skins of Nanorana yunnanensis which have pro-angiogenic ac-tivities.
