CAJ | 학술논문

目的：对灵杆菌素生产工艺进行优化，并对筛选工艺下产品的结构进行初步研究。方法：通过正交实验设计确定灵杆菌素最佳生产条件，并采用理化反应、TLC、HPLC等分析方法对产品的组成结构进行分析。结果：确定灵杆菌素最佳生产条件为：超声破壁时间为30min、苯酚溶液比例为1：30、5倍体积乙醇溶液沉淀。TLC 分析结果表明灵杆菌素是由甘露糖、半乳糖、葡萄糖和一种未知糖组成的多聚糖。HPLC 分析结果显示灵杆菌素具有明显的多糖特征峰，且其生产质量稳定。结论：科学筛选所确定的生产工艺条件能确保制备的灵杆菌素杂质含量少、质量稳定。
목적：대령간균소생산공예진행우화，병대사선공예하산품적결구진행초보연구。방법：통과정교실험설계학정령간균소최가생산조건，병채용이화반응、TLC、HPLC등분석방법대산품적조성결구진행분석。결과：학정령간균소최가생산조건위：초성파벽시간위30min、분분용액비례위1：30、5배체적을순용액침정。TLC 분석결과표명령간균소시유감로당、반유당、포도당화일충미지당조성적다취당。HPLC 분석결과현시령간균소구유명현적다당특정봉，차기생산질량은정。결론：과학사선소학정적생산공예조건능학보제비적령간균소잡질함량소、질량은정。
Objective:To optimize the production process and preliminarily study on product structure using the optimization pro-cess. Methods:To determine the optimization process conditions by Orthogonal experimental and To analyse product structure by the physical and chemical reaction, TLC,HPLC. Results:The optimization process conditions :the time of ultrasonic wall-breaking is 30min,the ratio of phenol solution is 1:30,the precipitation volume ethanol solution is five times; TLC results show that the Prodigiosin is kind of polysaccharide by mannose,galactose ,glucose and unknown sugar;HPLC results show that Prodigiosin presents obvious characteristic peak and production quality is stable. Conclusion: The optimization production process by selecting scientifically can sure less impurity and stable quality on Prodigiosin.