CAJ | 학술논문

目的测定哮喘小鼠中高敏C反应蛋白（hsCRP）的水平及其与气道炎症的相关性，观察气道炎症的抑制对其表达的影响。方法应用鸡卵白蛋白（OVA）致敏激发制备哮喘小鼠，测定哮喘小鼠血清、支气管肺泡灌洗液（BALF）中hsCRP水平，分析其与气道高反应性（AHR）、气道嗜酸细胞（Eos）和气道黏液分泌的相关性。在哮喘小鼠OVA激发前后分别腹腔注射DXM和anti-CD69mAb，观察它们对哮喘小鼠气道炎症及hsCRP的影响。结果哮喘小鼠血清hsCRP显著高于正常小鼠（P＜0.05），且与AHR、BALF中Eos、气管周围Eos浸润及黏液表达正相关（r分别=0.90、0.99、0.96、0.90，P均＜0.05）；但BALF中hsCRP未有显著增高。 DXM和anti-CD69mAb均可有效地抑制血清hsCRP的增高和哮喘小鼠的气道炎症（P均＜0.05）。结论哮喘小鼠存在以CRP为指标的系统性炎症，且其水平与气道局部炎症正相关，而气道炎症的抑制可有效地抑制系统性炎症。
목적측정효천소서중고민C반응단백（hsCRP）적수평급기여기도염증적상관성，관찰기도염증적억제대기표체적영향。방법응용계란백단백（OVA）치민격발제비효천소서，측정효천소서혈청、지기관폐포관세액（BALF）중hsCRP수평，분석기여기도고반응성（AHR）、기도기산세포（Eos）화기도점액분비적상관성。재효천소서OVA격발전후분별복강주사DXM화anti-CD69mAb，관찰타문대효천소서기도염증급hsCRP적영향。결과효천소서혈청hsCRP현저고우정상소서（P＜0.05），차여AHR、BALF중Eos、기관주위Eos침윤급점액표체정상관（r분별=0.90、0.99、0.96、0.90，P균＜0.05）；단BALF중hsCRP미유현저증고。 DXM화anti-CD69mAb균가유효지억제혈청hsCRP적증고화효천소서적기도염증（P균＜0.05）。결론효천소서존재이CRP위지표적계통성염증，차기수평여기도국부염증정상관，이기도염증적억제가유효지억제계통성염증。
Objective To assay the expression of high sensitivity C reactive protein (hsCRP) in asthmatic mice and its correlation to the airway inflammation, and observe the impact of the inhibition of airway inflammation on it. Methods Asthmatic mice were made by the sensitization and aerosol provocation of ovabulmin (OVA). hsCRP level in serum and bronchoalveolar lavage fluid (BALF) and their correlation to airway hyperrespoinsiveness (AHR), eosinophils and mucus overproduction were assayed. DXM and anti-CD69 mAb were administrated before and after OVA provocation, respective-ly, and their effects on airway inflammation and hsCRP expression were investigated. Results Serum hsCRP level in asth-matic mice was significantly higher than that in the normal mice (P<0.05), and positively correlated with AHR, eosinophils in BALF, peribronchial area, and mucus overproduction (r=0.90,0.99,0.96, 0.90,P<0.05). While hsCRP in BALF of asthmatic mice was at the similar level as normal mice. Treatment of DXM or anti-CD69mAb both inhibited the airway in-flammation, AHR and elevation of hsCRP level in serum effectively. Conclusion Systemic inflammation with the index of hsCRP existed in asthmatic mice, and correlated positively to the airway inflammation. The inhibition of airway inflamma-tion by anti-CD69 mAb could inhibit the systemic inflammation effectively.