CAJ | 학술논문

为获得带叶兜兰(Paphiopedilum hirsutissimum)种子萌发的共生真菌，采用原地共生萌发技术获得了2株自然萌发的小幼苗，并分离和筛选出了有效的种子萌发共生菌——瘤菌根菌(Epulorhiza sp.)。为验证分离菌株对带叶兜兰种子萌发的有效性，将Phs34号菌株与带叶兜兰种子在灭菌后的原生境基质上进行室内共生萌发试验，结果表明，经过6周的培养，对照组没有观察到种子的萌发；接菌的种子胚明显膨大，突破种皮，形成原球茎，平均萌发率为(58.35±3.41)%。这表明分离得到的瘤菌根菌能促进带叶兜兰的种子萌发。
위획득대협두란(Paphiopedilum hirsutissimum)충자맹발적공생진균，채용원지공생맹발기술획득료2주자연맹발적소유묘，병분리화사선출료유효적충자맹발공생균——류균근균(Epulorhiza sp.)。위험증분리균주대대협두란충자맹발적유효성，장Phs34호균주여대협두란충자재멸균후적원생경기질상진행실내공생맹발시험，결과표명，경과6주적배양，대조조몰유관찰도충자적맹발；접균적충자배명현팽대，돌파충피，형성원구경，평균맹발솔위(58.35±3.41)%。저표명분리득도적류균근균능촉진대협두란적충자맹발。
The aim was to obtain the compatible mycorrhizal fungi of lithophyticPaphiopedilum hirsutissimum usingin situseed baiting technique. Two natural small seedlings were harvested after in situ seed germination. The effective symbiotic fungi of genusEpulorhizawere isolated from these developed seedlings. In order to verify the effect of the isolated fungal strains on seed germination, one of strains, Phs34, was used to thein vitrosymbiotic seed germination ofP. hirsutissimumon the sterile habitat substrate. After cultured for 6 weeks, seeds inoculated with Phs34 started to develop into protocorms and average germination rate was (58.35±3.41)%, whereas seeds without the fungus failed to germinate. These indicated that the fungal strain Phs34 was compatible mycorrhizal fungus ofP. hirsutissimum, and it will be helpful in seedling production and orchid conservation ofP. hirsutissimum.