CAJ | 학술논문

为了解水稻(Oryza sativa)组蛋白去乙酰化酶HDA705的生物学功能，构建了HDA705酵母双杂交诱饵表达载体与双杂交文库，并筛选了与HDA705相互作用的蛋白。结果表明，HDA705的诱饵载体无自激活活性且对酵母无毒性作用，文库的滴度也适合常规的酵母双杂交文库筛选。通过对酵母双杂交文库的筛选，共获得了164个阳性克隆，经DNA测序分析，这些克隆编码47个可能与HDA705相互作用的蛋白，其中包括3个在逆境响应或激素信号转导过程中起到重要作用的(辅)转录因子、6个参与光合作用的叶绿体蛋白、1个含有R3H结构域的蛋白以及22种酶类等。这为进一步研究HDA705的生物学功能提供了重要的线索。
위료해수도(Oryza sativa)조단백거을선화매HDA705적생물학공능，구건료HDA705효모쌍잡교유이표체재체여쌍잡교문고，병사선료여HDA705상호작용적단백。결과표명，HDA705적유이재체무자격활활성차대효모무독성작용，문고적적도야괄합상규적효모쌍잡교문고사선。통과대효모쌍잡교문고적사선，공획득료164개양성극륭，경DNA측서분석，저사극륭편마47개가능여HDA705상호작용적단백，기중포괄3개재역경향응혹격소신호전도과정중기도중요작용적(보)전록인자、6개삼여광합작용적협록체단백、1개함유R3H결구역적단백이급22충매류등。저위진일보연구HDA705적생물학공능제공료중요적선색。
In order to understand the biological function of histone deacetylase HDA705 in rice, the yeast two-hybrid bait vector of HDA705 and the cDNA library were constructed and the interaction partners of HDA705 were screened. The results showed that HDA705 bait vector showed no self-activating and no toxicity to yeast cells. Furthermore, the titer of cDNA library was suitable for normal yeast two-hybrid screening assay. There were 164 positive clones by yeast two-hybrid screening. DNA sequencing analysis indicated that these positive clones encoded 47 proteins which may interact with HDA705. These proteins included 3 transcription factors (or transcription co-factors) which play key roles in stress responses and hormone signaling pathways, 6 chloroplast located proteins which were involved in photosynthesis, 1 protein containing the R3H domain structure, and 22 enzymes in plant, etc. These may provide important cues to further study the biological function of HDA705 in rice.