CAJ | 학술논문

为探讨细枝木麻黄(Casuarina cunninghamianaMiq.)愈伤组织分化过程的细胞组织学，对离体培养条件下的愈伤组织进行扫描电子显微镜和石蜡切片观察，分析愈伤组织的细胞分裂、分化以及芽再生的发生过程。结果表明，新鲜外植体培养于愈伤组织诱导培养基上，伤口处的薄壁细胞开始脱分化，培养1周后形成明显的愈伤组织；继续培养2周后，胚性愈伤组织形成，且表层细胞启动分化形成芽原基；培养4周，可肉眼观察到胚性芽原基，数量增多并逐渐分化形成不定芽；培养至第6周，生成不定芽，并大量增殖和分化。因此，细枝木麻黄是通过愈伤组织分化形成胚状体的途径进行植株再生的，为建立细枝木麻黄组织培养高效再生体系提供了理论依据。
위탐토세지목마황(Casuarina cunninghamianaMiq.)유상조직분화과정적세포조직학，대리체배양조건하적유상조직진행소묘전자현미경화석사절편관찰，분석유상조직적세포분렬、분화이급아재생적발생과정。결과표명，신선외식체배양우유상조직유도배양기상，상구처적박벽세포개시탈분화，배양1주후형성명현적유상조직；계속배양2주후，배성유상조직형성，차표층세포계동분화형성아원기；배양4주，가육안관찰도배성아원기，수량증다병축점분화형성불정아；배양지제6주，생성불정아，병대량증식화분화。인차，세지목마황시통과유상조직분화형성배상체적도경진행식주재생적，위건립세지목마황조직배양고효재생체계제공료이론의거。
In order to understand the cytohistology ofCasuarina cunninghamianacallus, cell division, callus differentiation and adventitious bud regeneration were studied under scanning electron microscope and parafifn sections. The results showed that parenchyma cells in the wound started dedifferentiation in the callus induction medium. After cultured for one week, callus were induced at the explant wound, and embryonic callus was formed. Bud primordium was induced from surface cells after two weeks. After culturing for a further four weeks, embryonic bud primordium became more increasely visible, and gradually differentiated to become adventitious bud. After another six weeks, most of the adventitious buds have proliferated and differentiated, and some developed to shoots. It was conifrmed that the regeneration ofC. cunninghamianacould be achieved through embryoid callus differentiation, it would provide basis for establishment of efifcient regeneration system inC. cunninghamina.