CAJ | 학술논문

目的：探讨灭幽汤治疗幽门螺杆菌(Hp)相关性胃炎脾胃湿热证的作用机制。方法将70只BALB/c小鼠随机分为对照组、模型组、胃三联组和灭幽汤高、低剂量组(中药高、低剂量组),每组14只,采用复合因素建立BALB/c小鼠Hp相关性胃炎脾胃湿热证模型。成模后各给药组给予相应药物,连续14 d,采用免疫组化检测Toll样受体(TLR)2、TLR4蛋白表达,实时荧光定量PCR检测TLR2、TLR4基因表达,ELISA检测血清白细胞介素(IL)-6、IL-8的表达。结果模型组TLR2、TLR4蛋白和基因表达水平及血清IL-6、IL-8含量均高于对照组(P＜0.01)；中药高、低剂量组和胃三联组TLR2、TLR4蛋白和基因表达及血清IL-6、IL-8含量均低于模型组(P＜0.01)；中药高剂量组TLR2、TLR4蛋白和基因表达水平及血清IL-6、IL-8含量虽低于胃三联组,但差异无统计学意义(P＞0.05)。结论灭幽汤可能通过抑制TLR2、TLR4及下游炎症因子的表达达到治疗Hp相关性胃炎脾胃湿热证的目的。
목적：탐토멸유탕치료유문라간균(Hp)상관성위염비위습열증적작용궤제。방법장70지BALB/c소서수궤분위대조조、모형조、위삼련조화멸유탕고、저제량조(중약고、저제량조),매조14지,채용복합인소건립BALB/c소서Hp상관성위염비위습열증모형。성모후각급약조급여상응약물,련속14 d,채용면역조화검측Toll양수체(TLR)2、TLR4단백표체,실시형광정량PCR검측TLR2、TLR4기인표체,ELISA검측혈청백세포개소(IL)-6、IL-8적표체。결과모형조TLR2、TLR4단백화기인표체수평급혈청IL-6、IL-8함량균고우대조조(P＜0.01)；중약고、저제량조화위삼련조TLR2、TLR4단백화기인표체급혈청IL-6、IL-8함량균저우모형조(P＜0.01)；중약고제량조TLR2、TLR4단백화기인표체수평급혈청IL-6、IL-8함량수저우위삼련조,단차이무통계학의의(P＞0.05)。결론멸유탕가능통과억제TLR2、TLR4급하유염증인자적표체체도치료Hp상관성위염비위습열증적목적。
Objective To investigate the mechanisms of Mieyou Decoction in the treatment of Hp-associated gastritis of spleen-stomach damp-heat syndrome. Methods Seventy BALB/c mice were randomly divided into control group, model group, high concentration of Mieyou Decoction group, low concentrations of Mieyou Decoction group, and gastric triad group, with 14 rats in each group. BALB/c mice of Hp-associated gastritis of spleen-stomach damp-heat syndrome models were established by composite factor. After the success of modeling and continuous 14-day administration, the expressions of TLR2, TLR4 protein, TLR2 mRNA, and TLR4 mRNA were detected by immunohistochemistry and qPCR. ELISA was used to detect the expressions of IL-6 and IL-8 in the serum. Results Compared with control group, the expressions of TLR2, TLR4 protein, mRNA, IL-6, and IL-8 in model group significantly increased (P<0.01);the expressions of TLR2, TLR4 protein, mRNA, IL-6, and IL-8 in high concentration of Mieyou Decoction group, low concentrations of Mieyou Decoction group, gastric triad group were lower than model group, with statistical significance (P<0.01);the expressions of TLR2, TLR4 protein, mRNA, IL-6, and IL-8 in high concentration of Mieyou Decoction group were higher than gastric triad group, but the differences were not statistically significant (P>0.05). Conclusion Mieyou Decoction may play a role in the treatment of Hp-associated gastritis of spleen-stomach damp-heat syndrome through intervention in the expressions of TLR2 and TLR4.