CAJ | 학술논문

目的：研究不同品种(系)黄芪和党参的遗传关系。方法对25个不同品种(系)黄芪和17个不同品种(系)党参进行 ISSR-PCR 扩增,用 Popgen32软件分析其遗传多样性及遗传距离,应用 NTSYS 软件构建 Nei's遗传距离UPGMA聚类。结果黄芪、党参样本分别检测出62、100个位点,多态位点百分率均为100%, Shannon信息指数平均值分别为0.5376、0.4727,Nei's基因多样度平均值分别为0.3613、0.3074；黄芪在遗传距离0.59、党参在遗传距离0.50处均被聚为2类。结论不同品种(系)的黄芪和党参在物种水平上具有较高的遗传多样性,且品种(系)间遗传差异较大。
목적：연구불동품충(계)황기화당삼적유전관계。방법대25개불동품충(계)황기화17개불동품충(계)당삼진행 ISSR-PCR 확증,용 Popgen32연건분석기유전다양성급유전거리,응용 NTSYS 연건구건 Nei's유전거리UPGMA취류。결과황기、당삼양본분별검측출62、100개위점,다태위점백분솔균위100%, Shannon신식지수평균치분별위0.5376、0.4727,Nei's기인다양도평균치분별위0.3613、0.3074；황기재유전거리0.59、당삼재유전거리0.50처균피취위2류。결론불동품충(계)적황기화당삼재물충수평상구유교고적유전다양성,차품충(계)간유전차이교대。
Objective To investigate the genetic relationships of Astragalus membranaceus and Codonopsis pilosula from the different cultivars (varieties). Methods Twenty-five samples of A. membranaceus and seventeen samples of C. Pilosula were amplified by ISSR-PCR. Genetic diversity and genetic distance were analyzed by Popgen32. UPGMA dendrogram relationship was clustered by NTSYS. Results Totally 62 and 100 loci were detected respectively from A. membranaceus and C. pilosula. All percentages of polymorphic loci (PPL) were 100%. Shannon’s information indexes (I) were 0.537 6 and 0.472 7. Nei’s genetic diversity indexes (H) were 0.361 3 and 0.307 4. A. membranaceus was divided into two groups in genetic distance 0.59, and C. pilosula was alike in 0.50. Conclusion Different cultivars (varieties) of A. membranaceus and C. pilosula had rich genetic diversities among species, and had large genetic differences.