CAJ | 학술논문

目的：对全国市售白芷饮片进行质量一致性的评价。方法采用高效液相色谱法(HPLC)测定12份不同地区的市售饮片、1份硫磺熏蒸药材和1份自然晾干药材的指纹图谱；测定饮片中欧前胡素和异欧前胡素的含量。HPLC条件为：C18色谱柱(4.6 mm×250 mm,5μm)；流动相为乙腈-水,梯度洗脱,流速1.0 mL/min；柱温35℃；检测波长选择210～800 nm范围的最大绘图。通过相似度计算方法进行分析比较。结果建立了白芷饮片的HPLC指纹图谱分析方法,确定了12个共有特征峰。市售饮片的相似度为0.840～0.973；与硫磺熏蒸药材相比,指纹图谱的相似度为0.672～0.908；与自然晾干药材相比,指纹图谱的相似度为0.536～0.684。市售饮片中欧前胡素和异欧前胡素的含量范围分别为0.035%～0.140%和0.028%～0.069%。结论不同地区的市售白芷饮片具有质量一致性,推测饮片均为硫磺熏蒸品。
목적：대전국시수백지음편진행질량일치성적평개。방법채용고효액상색보법(HPLC)측정12빈불동지구적시수음편、1빈류광훈증약재화1빈자연량간약재적지문도보；측정음편중구전호소화이구전호소적함량。HPLC조건위：C18색보주(4.6 mm×250 mm,5μm)；류동상위을정-수,제도세탈,류속1.0 mL/min；주온35℃；검측파장선택210～800 nm범위적최대회도。통과상사도계산방법진행분석비교。결과건립료백지음편적HPLC지문도보분석방법,학정료12개공유특정봉。시수음편적상사도위0.840～0.973；여류광훈증약재상비,지문도보적상사도위0.672～0.908；여자연량간약재상비,지문도보적상사도위0.536～0.684。시수음편중구전호소화이구전호소적함량범위분별위0.035%～0.140%화0.028%～0.069%。결론불동지구적시수백지음편구유질량일치성,추측음편균위류광훈증품。
Objective To evaluate the quality coherence of commercial Angelicae Dahuricae Radix (Baizhi) decoction pieces. Methods The samples of 12 batches of commercial decoction pieces, 1 batch of sulphur fumigated Baizhi, and 1 batch of naturally dried Baizhi were collected. HPLC method was used to determine the fingerprints and the contents of imperatorin and isoimperatorin by C18 column (4.6 mm×250 mm, 5 μm) with a gradient mobile phase of acetonitrile-water solution system at the flow rate of 1.0 mL/min. The column temperature was set at 35 ℃, and the max plot of detection wavelength was in 210-800 nm. Similarity calculation was used to analyze the data. Results The HPLC fingerprint analysis method was established with 12 common peaks. The similarities of 12 batches of commercial decoction pieces of Baizhi were 0.840-0.973. Their similarities compared with the sulphur fumigated Baizhi were 0.672-0.908. Compared with naturally dried Baizhi, the similarities of fingerprint were 0.536-0.684. The contents of imperatorin and isoimperatorin in 12 batches of commercial decoction pieces were 0.035%-0.140% and 0.028%-0.069%, respectively. Conclusion The quality of Baizhi decoction pieces was consistent. It can be speculated that Baizhi decoction pieces were processed with sulphur fumigation.