CAJ | 학술논문

目的：研究高浓度槲皮素对G6PD缺乏者体外红细胞的影响。方法选取G6PD活性正常、缺乏的检验者各50例,采集外周血并与槲皮素孵育,测定红细胞的GSH、MetHb,并对红细胞的形态学变化进行观察,考察槲皮素对G6PD活性缺乏者红细胞影响。结果槲皮素对G6PD缺乏者红细胞具有明显的氧化作用,GSH呈下降趋势,而MetHb则显著上升(P﹤0.05),高浓度组MetHb为(5.70±2.03)%显著高于中、低浓度组(3.51±1.67)%、(1.31±0.68)%,差异有统计学意义(P﹤0.05)；缺失组与正常组受到α-萘酚的影响存在明显差异,且两种孵育体系中的表现存在不同。结论槲皮素对G6PD缺乏者红细胞有一定的氧化作用,因此在涉及含有氧化性黄酮类化合物病制剂使用时应谨慎。
목적：연구고농도곡피소대G6PD결핍자체외홍세포적영향。방법선취G6PD활성정상、결핍적검험자각50례,채집외주혈병여곡피소부육,측정홍세포적GSH、MetHb,병대홍세포적형태학변화진행관찰,고찰곡피소대G6PD활성결핍자홍세포영향。결과곡피소대G6PD결핍자홍세포구유명현적양화작용,GSH정하강추세,이MetHb칙현저상승(P﹤0.05),고농도조MetHb위(5.70±2.03)%현저고우중、저농도조(3.51±1.67)%、(1.31±0.68)%,차이유통계학의의(P﹤0.05)；결실조여정상조수도α-내분적영향존재명현차이,차량충부육체계중적표현존재불동。결론곡피소대G6PD결핍자홍세포유일정적양화작용,인차재섭급함유양화성황동류화합물병제제사용시응근신。
Objective To study the effects of high concentrated quercetin on red blood cells in vitro of patients lacking G6PD. Methods A total of 50 cases whose G6PD activity were normal and 50 cases who lacked G6PD were selected. Peripheral blood was collected and incubated with quercetin to test the erythrocyte GSH and MetHb and the morpho-logical changes of red blood cells was observed. The effects of quercetin on erythrocyte of patients lacking G6PD activ-ity were investigated. Results Quercetin had obvious effect on the oxidation of erythrocytes of patients lacking in G6PD and the patients' GSH decreased but their MetHb increased significantly (P<0.05). The MetHb in the high concentra-tion group was (5.70±2.03)%, which was significantly higher than that in medium and low concentration groups (3.51± 1.67)%,(1.31±0.68)%. The difference was statistical significant(P<0.05). The effect of alpha naphtha on the patients in deficiency group and the normal group had obvious differences, but the performance of alpha naphthol in the 2 in-cubation system at the presence was different. Conclusion The effect of quercetin on red blood cells of erythrocyte of patients lacking G6PD have some adduction. So it could be cautious when the compounds preparation containing oxidiz-ing falconoid is used.