CAJ | 학술논문

目的：探讨ABI 7500与TL 988荧光定量PCR仪之间的性能差异。方法参照EP9-A2文件中的评价标准,两台仪器分别对5种不同浓度的甲型流感病毒阳性质控品进行检测,每个浓度测量20次,比对两种仪器的精密度、灵敏度,并评价两台仪器结果一致性。结果精密度：TL 988的变异系数(CV)从0.68%到1.52%,ABI 7500的CV从1.93%到2.69%。两台仪器在检测高浓度的甲型流感病毒DNA时(107、106、105 copies/mL)差异无统计学意义(P>0.05),检测低浓度的病毒DNA时(104、103 copies/mL)差异有统计学意义(P<0.05)。灵敏度：两种仪器检测浓度103 copies/mL时,TL 988阳性检出率为100%,ABI 7500检出率为0。两种仪器检测数据相关性良好(r=0.998)。结论荧光定量TL 988 PCR仪器的性能(精密度和灵敏度)比ABI 7500好。
목적：탐토ABI 7500여TL 988형광정량PCR의지간적성능차이。방법삼조EP9-A2문건중적평개표준,량태의기분별대5충불동농도적갑형류감병독양성질공품진행검측,매개농도측량20차,비대량충의기적정밀도、령민도,병평개량태의기결과일치성。결과정밀도：TL 988적변이계수(CV)종0.68%도1.52%,ABI 7500적CV종1.93%도2.69%。량태의기재검측고농도적갑형류감병독DNA시(107、106、105 copies/mL)차이무통계학의의(P>0.05),검측저농도적병독DNA시(104、103 copies/mL)차이유통계학의의(P<0.05)。령민도：량충의기검측농도103 copies/mL시,TL 988양성검출솔위100%,ABI 7500검출솔위0。량충의기검측수거상관성량호(r=0.998)。결론형광정량TL 988 PCR의기적성능(정밀도화령민도)비ABI 7500호。
Objective To explore the differences between ABI 7500 and TL 988 fluorescent quantitative instrument for polymerase chain reaction. Methods Based on EP9-A2 Files, quantitative influenza A virus positive controls of five kinds of concentrations were detected by two kinds of fluorescence quantitative PCR instruments at the same time, and each sample was detected 20 times. The precision and sensitivity between the two kinds of instruments were compared. Results Inner group CV of TL 988 ranged from 0.68% to 1.52%. The CV for ABI 7500 ranged from 1.93% to 2.69%. The high (107, 106, 105 copies/mL) levels of IFVA positive control were detected by ABI 7500 and TL 988 instruments, and there were no significant difference between the results(P>0.05) while the low(104, 103 copies /mL)levels were de-tected by ABI 7500 and TL 988 instruments, and there was a significant difference between the results(P<0.05). When samples were detected at the level of 103 copies/mL, the positive detection rate was 100% with TL 988 instrument, but the positive detection rate was 0 with ABI 7500 instrument. The related index of ABI 7500 and TL 988 was better (r=0.998). Conclusion The performances in precision and sensitivity of TL 988 PCR instrument are better than ABI 7500 PCR.