CAJ | 학술논문

目的：探讨可溶性Aβ1－42寡聚体双侧海马CA1区注射是否对Wistar大鼠认知功能具有损害作用。方法：雄性 Wistar大鼠40只随机分为空白对照组（Intact group）、假手术组（Sham group）、溶剂对照组（Vehicle group）、Aβ组（Aβgroup）4组，空白对照组不做任何处理，假手术组仅行双侧海马CA1区插管，溶剂对照组对双侧海马CA1区注射与Aβ组等体积的灭菌双蒸水（dd H2O），Aβ组给双侧海马CA1区注射可溶性Aβ1－42寡聚体；注射后两周用Morris水迷宫检测各组行为学表现。结果：Aβ注射可以明显增加大鼠逃避潜伏期与上台前路程（ P ＜0．01），对大鼠平均游泳速度无影响，而Intact、Sham、Vehicle组之间差异无统计学意义（ P ＞0．05）；空间探索实验结果显示，Aβ注射可明显减小大鼠目标象限时间百分比（ P ＜0．01）与穿越平台次数（ P ＜0．05）。结论：双侧海马 CA1区注射可溶性Aβ1－42寡聚体对大鼠认知功能有明显损害作用。
목적：탐토가용성Aβ1－42과취체쌍측해마CA1구주사시부대Wistar대서인지공능구유손해작용。방법：웅성 Wistar대서40지수궤분위공백대조조（Intact group）、가수술조（Sham group）、용제대조조（Vehicle group）、Aβ조（Aβgroup）4조，공백대조조불주임하처리，가수술조부행쌍측해마CA1구삽관，용제대조조대쌍측해마CA1구주사여Aβ조등체적적멸균쌍증수（dd H2O），Aβ조급쌍측해마CA1구주사가용성Aβ1－42과취체；주사후량주용Morris수미궁검측각조행위학표현。결과：Aβ주사가이명현증가대서도피잠복기여상태전로정（ P ＜0．01），대대서평균유영속도무영향，이Intact、Sham、Vehicle조지간차이무통계학의의（ P ＞0．05）；공간탐색실험결과현시，Aβ주사가명현감소대서목표상한시간백분비（ P ＜0．01）여천월평태차수（ P ＜0．05）。결론：쌍측해마 CA1구주사가용성Aβ1－42과취체대대서인지공능유명현손해작용。
Objective:To explore whether soluble Aβ1-42 oligomers injection could impair cognitive performance in Wistar rats .Methods:Forty male rats were randomly divided into four groups:Intact group, Sham group, Vehicle group and Aβgroup.Two weeks after the injection of soluble Aβ1-42 oligomers, the Morris water maze (MWM) test was performed to analyze the effect of soluble Aβ1-42 oligomers on cognitive per-formance in rats.Results:The escape latency and path length of rats was significantly prolonged in Aβgroup ( P <0.01), without significant effect on mean swim speed.There was no significant difference among the Intact、Sham and Vehicle group.In the probe trails, soluble Aβ1-42 ol-igomers injection could significantly decrease the time percentage in the target quadrant ( P <0.01)and cut down the times in crossing platform ( P <0.05).Conclusion: Soluble Aβ1-42 oligomers injection can bring about impairment to the cognitive performance in rats.